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The following parameters were used for the T1-weighted coronal spin-echo sequence: TR/TE 450/20; matrix size 256��192; section thicknes 3 mm; FOV 16 cm; bandwidth 16. The following parameters were used for the stir coronal sequence: TR/TE 746/7, matrix size 256��192; section thicknes 4 mm; FOV 16 cm; bandwidth 19 cm. The size of the patchy areas compatible with residual red bone marrow in the distal femoral metaphyses, hypointense relative to the neighboring bone tissue but hyper-isointense relative to the neighboring muscle tissue in T1WS, were evaluated qualitatively. Those areas were confirmed as being hyperintense in the STIR sequence. The lesions that are more hypointense than the neighboring muscle tissue are accepted as pathological (malignancy etc.) and were not included in the study. The measurements were repeated twice at different times in order to increase the accuracy of the grading system. The patchy areas [http://en.wikipedia.org/wiki/Unoprostone Unoprostone] were defined as grade 1 if mild and covering less than 30% of the metaphysis, grade 2 if moderate and covering between 30% and 60% of the metaphysis, and grade 3 if diffuse and covering more than 60% of the metaphysis (Figures 1, ?,22). Figure 1 The T1WS coronal sequence of knee MRI [http://www.selleckchem.com/products/bgj398-nvp-bgj398.html selleck products] in a 36-year-old female weighing 80 kg. The residual red bone marrow areas were of grade 1 (Hgb level: 12.9 g/dL). Figure 2 The T1WS coronal sequence of knee MRI of a 36-year-old female weighing 66 kg. The residual red bone marrow areas were of grade 3 (Hgb level: 15.1 g/dL). Exclusion criteria Cases under the age of 25 (since the adult bone marrow pattern is not formed until then); Cases who smoked (8); Athletes; Patchy areas which were hypointense when compared with the neighboring muscle tissue [9]; Cases with marrow edema due to contusion; History of steroid use, osteonecrosis and fracture; Cases with malignant or systemic diseases that might have affected the bone marrow. Statistical analysis The statistical analysis in this study was carried out using the NCSS (Number Cruncher Statistical System) 2007 Statistical Software (Utah, USA) package program. In [http://www.selleckchem.com/screening/protease-inhibitor-library.html Protease Inhibitor Library price] evaluating the data, the descriptive statistical methods (average, standard deviation) were used. The One-Way Analysis of Variance was used in comparisons among the groups; the Tukey��s Multiple Comparison Test was used in comparisons of the sub-groups; and the Chi-Square Test was applied to compare the qualitative data. In the differential diagnosis of signal increase for hemoglobin, the area under the ROC curve, sensitivity, specifity, positive and negative predictive value, LR (likelihood ratio) (+) value and predictive value were measured. The results were evaluated at significance level of P