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1% PBST?+?1% skim milk and incubated at 4?��C overnight. After rinsing in PBST the membranes were incubated with fluorescent secondary antibodies anti-goat IRDye (1:5000) and anti-mouse Alexa Fluo (1:5000) (LI-COR) diluted in PBST?+?1% milk powder for 2?h at room temperature. The detection and quantification [http://www.selleckchem.com/products/nlg919.html NLG919] of the signal was performed by the Odyssey? infrared detection system (LI-COR). Data of body weight, blood glucose levels, protein quantification and activity of acetylcholinesterase were evaluated using the Student's t test. Analysis of variance of one or two ways followed by Tukey's test was also used for activity assays. The larger number of pups per litter to induce protein-energy malnutrition caused significant reduction compared to the control group in body weight of malnourished animals at weaning. Body weight in this period was: weaning, 35.50?��?5.16?g control, 28.00?��?2.00?g, malnourished P?[http://en.wikipedia.org/wiki/Tubulin Tubulin] when compared with the control animals (1.245?��?0.025?g) P?[http://www.selleckchem.com/products/Verteporfin(Visudyne).html Verteporfin molecular weight] of acetylcholinesterase in malnourished and control animals was performed in order to express the enzyme activity in U/mL (Table 1) and its specific activity in U/mg protein (Fig. 2). The differential AChE activity found in HC homogenate between control and malnourished rats reflects diluted fractions whilst HFCT characterize membrane-bound and diluted fraction of AChE enzyme. Analysis showed a significant difference among the 3 different types of homogenates in the control (P?