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Measurements of salivary sIgA Subjects rinsed their mouth twice and gargled twice with 150 ml of water for each time before testing. After sitting for 15 min, subjects [http://www.selleckchem.com/products/Verteporfin(Visudyne).html Verteporfin price] underwent sampling of their saliva using a Salisoft (Sarstedt, Numbrecht, Germany) for 120 sec. The amount of saliva recovered was recorded, and the salivary concentration of sIgA was determined by immunonephelometry according to the standard laboratory protocol of the BML Inc. (Tokyo, Japan). T-cell subset analysis The cell surface CD4 and CD8 antigens on peripheral blood lymphocytes were analyzed by flow cytometry using fluorescent-labeled monoclonal antibodies specific for the respective cell surface antigens (cat. nos. 340133 and 347313, respectively; BD Biosciences, Tokyo, Japan) according to the standard laboratory protocol of the LSI Medience Corporation (Tokyo, Japan). Statistical analysis All the measured values are expressed as the mean �� standard error, or as indicated, the error bars may represent the standard error of the mean. For comparison of the values in the placebo and active food groups, ��2 test (for [https://en.wikipedia.org/wiki/Itraconazole Itraconazole] gender) or unpaired Student's t-test (for others) were employed, and P[http://www.selleckchem.com/products/Vandetanib.html find more] in group P excluded themselves from the study at day 4. The remaining 59 participants completed the study. However, 5 subjects were judged to fall into exclusion criteria and were eliminated from the efficacy analysis for the following reasons: Diagnosed as diabetes mellitus (n=1 in group A), lifestyle change due to hospitalization for lumbar disc herniation (n=1 in group A), and long-term cold that may affect sIgA release (n=2 in group A and n=1 in group P). Thus, the data obtained with 54 participants (n=26 in group A and n=28 in group P) were used for the efficacy analysis (Fig. 1). Background data The background data of the participants who were subjected to efficacy analysis are shown in Table II. No significant difference was observed in the values between groups A and P for each item, including the rate of salivary sIgA release and T-cell subset population. Table II. Background characteristics of the subjects. Effects of black-vinegar-mash-garlic-containing food intake In group A, the rate of salivary sIgA release was significantly high (P=0.028) compared to that in group P at week 4 of the study (Fig. 2). The rate in group A at week 8 was also higher than that in group P.