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A descriptive analysis was carried out comparing the experimental groups and assessing the occurrence and pattern of newly formed bone tissue. Histomorphometric analysis to determine the volume density of bone tissue, connective tissue and newly formed vessels was performed on the whole central region of the CSD (digital images of non-superimposed contiguous fields, six per slide, ��170 magnification) using the Image Pro-Plus 6.0? software (MediaCybernetics), previously calibrated in micrometers/pixel and with [http://www.selleckchem.com/products/Metformin-hydrochloride(Glucophage).html Metformin] a superimposed 100 points grade. This procedure was performed by two calibrated independent examiners, which means the maximum variation was less than 0.01% (paired t-test; InStat 3.01?, GraphPad). The means and standard deviations obtained were submitted to variance analysis (ANOVA) using InStat 3.01? and Prism 5.0? softwares (GraphPad), defining significant differences if P? standard deviations of groups were significantly different the nonparametric Kruskal�CWallis test was applied. The radiographic images obtained showed a pattern of bone growth from the edges of the CSD, irregular in their distribution. There was a strong change in optical density represented by shades of gray in the same group of animals tested, as well as the presence of islets with higher optical density suggesting new bone. The variation in the area of newly formed bone was regular in GI in opposition to GII, which had a significant increase in the bone area when compared 2 weeks to 4 weeks (2.5 times; P?[http://www.selleckchem.com/products/isrib-trans-isomer.html ISRIB clinical trial] In relation to the effect of treatment between the groups, GI and GII had significant difference only at 8 weeks (P?[http://en.wikipedia.org/wiki/PSCD4 CYTH4] vessels of large and small caliber and reversal lines was noted in both groups (Fig. 3A and B). At 4 weeks, GI showed trabecular bone formation with a high number of osteocytes and cortical bone formation in margins of the CSD, while in GII there was a larger amount of bone formation with a greater presence of compact bone in the periphery and few trabecular spaces. In this group the bone growth was more pronounced and obvious toward the center of CSD with the formation of islets of bone tissue than bone growth toward the endosteum and periosteum, with characteristics of mature (cortical) bone (Fig. 4A and B). At 8 weeks, presence of newly formed bone with few marrow spaces, and a moderate amount of reversal lines in GI (Fig. 5A) with few osteoblasts and occasional presence of osteoclasts.