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Bivariate partnership of line suggests for net in vivo ROS level and 8-oxodG content material. Relative reactive oxygen species (ROS) levels are reported in relative fluorescence units (RFU); quantity of 8-oxo-7,8-dihydro-29-deoxyguanosine, or 8-oxodG, are reported as 6109 broken bases per nanogram of DNA. Line suggests of your two metrics were considerably correlated (Spearman's r = 0.943, p = 0.017 with all lines present). Bars represent 1 typical error. ``N2 AC'' is definitely the N2 ancestor (progenitor of MA lines, Generation 0); remaining data labels would be the Baer MA line numbers. doi:10.1371/journal.pone.0065604.gthe MA atmosphere on 8-oxodG is practically sufficiently massive (0.0028/generation; p,0.1) and has precisely the same magnitude of the per-generation alter as ROS level, consistent with all the quite higher correlation of line implies amongst the two variables (Spearman's r.0.9). The tiny number of lines raises the concern that any statistically substantial outcome is actually a false positive. Though that possibility can't be ruled out, we note that two on the MA lines we attempted to assay had such low fitness that we had been unable  to conduct the assays. Since the lines we had been able to assay are Table 1. Estimates of oxidative pressure and mutation frequency.Line{ 523 526 529 553 574 MA mean N2 ancestorRelative ROS (SE)` 471.4 (75.8)* 269.2 (57.3) 282.8 (49.2) 261.5 (48.6) 350.4 (38.4)* 328.2 (39.6) 192.9 (29.6)8-oxodG (SE)1 mBS" 54.92 (7.4)* 35.95 (4.6) 33.72 (1.1) 26.43 (7.8) 50.79 (10.4)* 40.72 (5.4) 23.97 (8.7) 3.163E-09 2.446E-09 1.845E-09 2.890E-09 1.757E-09 ??mG-TO-T 3.987E-05 1.417E-05 2.080E-05 3.687E-05 1.982E-05 ??{ Baer mutation accumulation (MA) line number from the Baer et al. (2005) experiment [28]. ` Relative reactive oxygen species (ROS) levels expressed as means (standard error) of relative fluorescence units. *Indicates significantly [https://www.medchemexpress.com/EPZ-6438.html EPZ-6438 biological activity] different from N2 ancestor. 1 Means (standard error) of 8-oxo-7,8-dihydro-29-deoxyguanosine, or 8-oxodG, are reported as 6109 damaged bases per nanogram of DNA. " See Materials and Methods for calculations of point estimates of the frequencies of base substitutions (mBS) and G-to-T transversions [http://www.ncbi.nlm.nih.gov/pubmed/ 23148522  23148522] (mG-TO-T). doi:10.1371/journal.pone.0065604.tupwardly biased relative to a random sample of MA line fitnesses and because increased susceptibility to oxidative stress is commonly associated with low fitness [58,59], there is at least some reason to believe that the results of these assays are likely to be conservative compared to the results of an assay of MA lines randomly sampled with respect to fitness. In contrast to the strong association of the two measures of oxidative stress with the MA environment, there is no strong association between either oxidative stress measure and the total frequency of base substitutions (mBS) or G:C to T:A transversions (mG-to-T). Although the results of this study and results from other studies involving C.