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For ASC on PLGA 70 fields of view (FOV) were analyzed and for PLGA/a-CaP 80 FOV were analyzed. Extracellular matrix (ECM) production was evaluated in the Masson Goldner Trichrome stained sections as the percentage of the whole scaffold area in 16 FOV of PLGA and 16 FOV of PLGA/a-CaP at 200�� magnification using analysis^D software. The data were analyzed with StatView 5.0.1. Statistical analysis of variance (ANOVA) was conducted to test the significance of differences between different time points (1?week versus 2?weeks) used for the quantification of ASC numbers on PLGA/a-CaP [http://www.selleckchem.com/products/ABT-888.html selleck inhibitor] tubes, for the ASC width as well as the ECM production on different scaffold materials. Pairwise comparison probabilities (p) were calculated [https://en.wikipedia.org/wiki/PDE4B PDE4B] using the Fisher's PLSD and the Bonferroni post hoc test. P values [http://www.selleckchem.com/products/EX-527.html find more] during the incubation period (Fig. 2c and d). The width of the cells were found to be significantly different with 4.6?��?1.7?��m (n?=?70) for ASC on PLGA and 3.5?��?1.7?��m (n?=?80) for ASC on PLGA/a-CaP after one week with p?