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Virophages infect the virus production factories of NCLDV viruses during Eukaryotic infection (La Scola et al., 2008). They represent a type of satellite virus, having a negative impact on NCLDV viruses, and even allowing for recovery of their shared hosts in the case of Sputnik virophage, the NCLDV mamavirus and their Acanthamoeba host (La Scola  et al., 2008). The cryoconite virophage genome scaffold contained gene [http://www.selleckchem.com/products/ldk378.html Ceritinib] homologs to Sputnik and Yellowstone Lake Virophage, suggesting an Amoeboid host, therefore its presence points toward a complex interplay between Eukaryotic hosts and their viruses in cryoconite. To summarize, cryoconite holes possess simple truncated ecosystems, however, the interaction between viruses, their hosts and other competing viruses is complex. Almost all the viruses we have assembled in this study are novel, forming multiple novel virus groups that suggests multiple life strategies and interactions are taking place. These include lysis, lysogeny, plasmid replication with host death-on-curing, satellite phage plasmids which rely on other lysogenic phage for horizontal transmission and even viruses parasitizing on other virus infections. The identification of a phage encoding a bacterial immune system also suggests that some lysogenic phage, in their effort to survive phage competition, may even be beneficial to the bacterial host, providing immunity against other lytic phage infections. This study has highlighted the benefit of assembling virus genomes from environmental samples  to complement virus metagenomics, particularly in novel habitats where the majority of viruses are unknown. By focusing in on the dsDNA viruses, we have demonstrated that this approach is also suitable for challenging samples where cellular DNA contamination is present. Conflict of interest statement The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Acknowledgments This work was funded by both the Leverhulme Trust (RPG-2012-624) to AA and CB and the National Environment Research Council (NE/ J013854/1) to AA and CB. We would like to thank Laura Sanguino, Catherine Larose and Timothy Vogel from the Environmental Microbial Genomics Group, Laboratoire  Amp��re, for discussing early bioinformatics analysis ideas and an introduction to CRISPRs. We would also like to thank Declan Schroeder from the Marine Biological Association of the UK for laboratory time and help with virus concentration. We also thank Jane Coghill and Christy Waterfall from the Bristol Genomics Facility for their help, advice and excellent service. Supplementary material The Supplementary Material for this article can be found online at: http://journal.frontiersin.org/article/10.3389/fmicb.2015.00656 Click here for additional data file.(747K, DOCX) Click here for additional data file.