Перегляд вихідного коду сторінки I'd Believe You Also Make These Kinds Of Mistakes With MDV3100 ?

From electrophoretic mobilities, the electrostatic potential at the shear plane, denoted as �� ? potential, is usually determined using either the Smoluchowski or the Henry equation ( 23). From �� ? and for 1:1 electrolytes, the surface charge �� ? is deduced using the Gouy or Grahame equation equation(2) ��=(8cel�Ŧ�0kBT)1/2sinh(e��02kBT)�Ԧ�TH,where [http://www.selleckchem.com/products/MDV3100.html Selleckchem MDV3100] e ? is the proton charge and ��0��0 denotes the surface potential and it is assumed that ��0=�Ʀ�0=��. Here, celcel denotes the electrolyte concentration, kBkB the Boltzmann constant, and T ? the temperature. Equation 2 implies that at a molecular scale equation(3) ��=��zczs��(z��)dz��Ԧ�MDholds. Here, z��z�� is the position normal to the membrane, with zczc residing in the center of the membrane, and zszs at the shear plane. The symbol �� ? denotes the charge density from Na+ and Cl? ions. To check whether the surface [http://www.selleckchem.com/products/AZD6244.html selleck compound] charges from Eqs. 3 and 2 are consistent we have used both equations to estimate the surface charge for the POPC bilayer studied in our recent MD simulations (V. Knecht, B. Klasczyk, R. Dimova, unpublished). To this aim, the electrophoretic mobility observed in the simulations, ��E,MD��E,MD, was converted into a zeta potential using the Helmholtz-Smoluchowski equation, leading to ��=28.2(��2.1)��=28.2(��2.1) mV. Equation 2 yields ��TH=0.255(��0.020)��TH=0.255(��0.020)e ?/nm2. The corresponding surface charge from Eq. 3 skipping the initial 20?ns for equilibration [http://en.wikipedia.org/wiki/Telomerase Telomerase] was ��MD=0.263(��0.017)��MD=0.263(��0.017)e ?/nm2. Hence, within the statistical uncertainty ��TH=��MD��TH=��MD, that is, the surface charges from Eqs. 3 and 2 are consistent. Thus, plugging �� into Eq. 2 yields a correct estimate for ��. On the other hand, from �� ? obtained via Eq. 2 and if the membrane is neutral in the absence of ions, the fraction of hydrodynamically bound ions, ��hyd��hyd, may be deduced according to equation(4) ��hyd=�Ҧ�1,with ��1=q/alip��1=q/alip where alipalip denotes the area per lipid and q ? the charge of the surface-active ions. Experiments yield is alipalip?= 0.683?nm2 for POPC ( 24). We note that whereas NaCl leads to a decrease in alipalip in MD simulations it does not affect alipalip significantly for NaCl concentrations below 1 M experimentally. According to Eq. 3, Eq. 4 implies that ��hyd��hyd obeys equation(5) ��hyd=alip��zczsn(z��)dz��,with n(z��)=n+(z��)+n?(z��)n(z��)=n+(z��)+n?(z��), where n+(z��)n+(z��) and n?(z��)n?(z��) denote the local concentrations of cations and anions, respectively. If only cations are hydrodynamically adsorbed, n?(z��)=0n?(z��)=0 and n(z��)=n+(z��)n(z��)=n+(z��) for z