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The time elapsed from entering [http://www.selleckchem.com/products/Dasatinib.html selleck screening library] the canal with the first instrument until no obturation material was seen covering the instruments was measured (T1?+?T2). The numbers and types of fractured and deformed instruments were recorded. The roots were vertically split into halves using diamond burs and a spatula. After vacuum drying, the specimens were sputter coated with gold palladium under a 10?mA current. Micrographs at magnifications of 500�� and 1500�� were then taken under a scanning electron microscope at 25?kV. The residual obturation material and debris at the coronal, middle and apical thirds of each canal were evaluated using the H��lsmann [13] grading system. The H��lsmann system comprises the following grades: Canal walls are clean with a slight presence of debris. Some presence of debris. Presence of debris in 50% of the canal walls. Nearly all of the canal walls (90�C100%) are covered with debris. Statistical analysis was performed using the Number Cruncher Statistical System 2007 and PASS 2008 Statistical Software programme. Intergroup comparison was performed using one-way analysis of variance and Kruskal�CWallis tests. Tukey's honestly significant difference test was performed to identify significant differences among groups. The level of significance was set at P?[http://en.wikipedia.org/wiki/Oxygenase Oxygenase] observed in all groups. Specimens prepared with Mtwo instruments showed significantly more remaining filling material at the coronal third of the roots than specimens prepared with ProTaper (P??0.05; Table?1). T1, T2 and Tt of the teeth prepared with ProTaper were found [http://www.selleckchem.com/products/Gefitinib.html selleck kinase inhibitor] to be significantly less than those of teeth prepared using Mtwo, irrespective of obturation and retreatment techniques (P?