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Later, all the snakes were placed under hibernation conditions (6��C) during 3 months. Finally all the snakes were placed in the optimal thermal regime during 2 additional months. The total duration of the experiment was of 12 months. Body mass and feeding rates were recorded weekly, body size was measured monthly. We also assessed locomotor performance and recorded several behavioral traits (e.g., defensive and predatory behaviors). As expected, snakes raised under cool temperatures [http://www.selleckchem.com/products/mi-773-sar405838.html selleck chemicals] exhibited low feeding rate, growth rate, body condition, and they exhibited poor locomotor performance; they also displayed marked defensive behaviors (e.g., high number of defensive bites) whilst hesitating during longer periods to bite a prey. Such behavioral effects were detected at the end of the experiment (i.e., 5 months after exposure to contrasted thermal treatments [3 months of hibernation plus 2 months of optimal regime]), revealing long term effects. Surprisingly, growth rate and locomotor performance were not different between the two other groups, warm constant 28��C versus optimal regimes (albeit several behavioral traits differed), suggesting that the access to a wide range of ambient temperatures was not a crucial factor. J. Exp. Zool. [http://www.selleckchem.com/products/lee011.html Selleckchem LEE011] 319A:310�C318, 2013. ? 2013 ? 2013 Wiley Periodicals, Inc. ""Small vesper mice (Calomys laucha) may be considered as an animal model for in vitro fertilization studies, but limited data about in vitro evaluations of their sperm quality and fertility are available. The in vitro penetration [http://en.wikipedia.org/wiki/Resiquimod Resiquimod] (IVP) assay is used to estimate potential sperm fertility for many mammal species, but it still requires reduction in cost and labor. This study tested improvements in the IVP assay for C. laucha sperm using swine oocytes and perivitelline layers (PVL) of chicken eggs as substrates, and evaluated associations among C. laucha sperm quality, IVP, and in vivo fertility after natural mating. In the IVP assay, gametes coincubation was carried out flat-bottomed wells with M2, in water bath at 37��C for 2?hr. C. laucha sperm presented motility, normal morphology, membrane integrity, and acrosome integrity equal to 90.6 �� 5.6, 90.2 �� 6.6, 88.7 �� 9.6, and 90.5 �� 11.5%, respectively. The IVP rate was 39.8% in swine oocytes and 87.5% in the inner PVL. Considering in vivo fertility as the gold standard, the IVP assay in swine oocytes presented a sensitivity of 16.0% and specificity of 83.3%. The sensitivity of the IVP assay in the inner PVL was 84.0%, but the specificity was not determined because there were no true negative results. Sperm membrane integrity was correlated with parturition after natural mating (r = 0.38, P