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Twenty-five children (66%) reacted with positive and 13 children (34%) with negative DBPCFC (Table?2). Of the 25 reacting, ten claimed they had never eaten peanuts, and of the 13 nonreacting children, eight had the same claim while five reported a previous allergic reaction to peanut. The symptoms were scored according to Astier et?al. (6) and were mild to moderate (grade 1�C3) in fifteen (60%) of the reacting children. Nine children (36%) had severe reactions (grade 4), and one child (4%) was observed in the intensive care unit (grade 5) (Table?2). No child reacted to placebo. No association was found between the threshold dose of peanut at challenge and the severity of the reaction (rs?=?0.21, P?=?0.32) (Table?2). Ninety-two per cent (22 of 24) of the children with a positive [http://www.selleckchem.com/products/Everolimus(RAD001).html Everolimus] DBPCFC were positive in CD-sens after stimulation with peanut and 92% (23 of 25) after [https://en.wikipedia.org/wiki/Cilengitide Cilengitide] stimulation with Ara h 2 (Table?3) (Fig.?1). Two children were low responders, having a low response to the positive control, anti-Fc��RI, ([http://www.selleckchem.com/products/PD-0332991.html Palbociclib cost] in CD-sens, but the CD63 expression barely reached the 5% cut-off; therefore, a CD-sens value could not be calculated. The median peanut serum IgE-ab level in the whole group at the time of challenge was 36.5 (range 0.1�C1022)?kUA/l. Children positive at DBPCFC had significantly higher levels of peanut IgE-ab, 114 (range 1.0�C1022)?kUA/l, compared with those negative at the DBPCFC, 1.0 (range 0.1�C46.9)?kUA/l (P?