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Data showed non-parametric distribution & Mann�CWhitney U test was used for comparisons between two groups. This test is the non-parametric alternative to Student��s t-test. Chi-square (��2) test was used for studying the comparisons and associations between different qualitative variables. Spearman��s correlation coefficient was used to determine significant correlations between CEBPA expression and different variables. Kaplan�CMeier survival curve was constructed for survival analysis. The significance level was set at P???0.05. Statistical analysis was performed with IBM/SPSS Statistics Version 20 for Windows. Characteristics of AML patients are shown in (Table 1). Results of morphological examination, cytochemical staining and immunophenotyping performed on peripheral blood and bone marrow samples showed that the [http://www.selleckchem.com/products/nu7441.html NU7441 solubility dmso] most common subtype of AML in our study population according to the FAB classification was 19(47.5%) cases of M3. Nine cases (22.5%) of M1, 5 cases (12.5%) of M5b, 2 cases (5%) of M5a, 2 cases (5%) of M0, 2 cases of M2 (5%) and 1 case (2.5%) of M6. No M7 [http://en.wikipedia.org/wiki/ATP7A ATP7A] was seen in our study. To quantify the relative expression of CEBP�� mRNA, the Ct (threshold cycle) values were normalized for endogenous reference (��Ct?=?Ctsample_CtGAPDH) and compared with a calibrator, using the ��delta�Cdelta Ct method�� (����Ct?=?��CtSample_Ctcontrol). As calibrator we used the average CEBP�� mRNA Ct value in the 20 P.B and B.M samples of healthy volunteers. Using the ����Ct value, the relative expression was calculated (2?����Ct) [14]. CEBP�� mRNA expression results were classified into two subgroups: Low and High expression according to CEBP�� mRNA expression mean level of the healthy population. CEBP�� mRNA expression in the healthy population had a mean of 0.97 (considered as cutoff value) using the ����Ct method. Low values below the control mean (0.97) was considered ��High�� expression. The results obtained using this method showed low expression of CEBP�� mRNA in 29 (72.5%) out of the forty patients (values of CEBP�� mRNA expression were below the cutoff) with median level of 0.19 (range 0�C0.87) while the other eleven [http://www.selleckchem.com/screening/anti-infection-compound-library.html Antiinfection Compound Library] patients (27.5%) had higher expression of CEBP�� mRNA (values were above the cutoff) with a median of 1.52 (range 1.07�C2). In cases with all FAB subtypes & M3 subtype; there was a statistically significant difference between cases with low and higher CEBPa mRNA expression (P-value?=?