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?coli cells/mL and more than 105 leukocytes/mL, five patients with urine cultures with more [http://en.wikipedia.org/wiki/PRDX5 PRDX5] than 105P.?aeruginosa cells/mL and more than 105 leukocytes/mL, and seven patients with blood cultures and four with cultures of other fluids (articular, pleural and perioperative) yielding Staphylococcus aureus or Enterobacteriaceae. To the best of our knowledge, this is the first study concerning the evaluation of the usefulness of an NS providing bacterial identification and AST. A laboratory organization providing such a continuous service with personnel specifically dedicated to microbiology is resource-consuming and must therefore be justified. Most previous reports concerning the impact of after-hours Gram stain examination and/or inoculation [http://www.selleckchem.com/products/mcc950-sodium-salt.html MCC950 in vivo] of agar media with positive blood cultures have demonstrated a benefit for patients and a benefit with respect to antimicrobial resistance. Similarly, the importance of the presence of personnel specialized in the field of bacteriology was confirmed recently by a study reporting the consequences of errors in interpretation of Gram stains from positive blood cultures [15] and by another study showing an association between prompt Gram staining of blood cultures and decreased mortality [16]. The main result of our 4-month study was the demonstration of a 111-day benefit of early appropriate treatment and a 27-day benefit of reduced-spectrum but still efficient antibiotic regimens as a consequence of the NS activity. Fraser et?al. [17] identified a benefit of appropriate empirical antibiotic treatment concerning 30-day mortality and duration of hospital stay. In this study, 30-day mortality rates were 20.1% and 11.8% in patients who received inappropriate and appropriate treatment, respectively (p?[http://www.selleckchem.com/products/midostaurin-pkc412.html buy Midostaurin] stay was at least 2?days longer for patients who received inappropriate antibiotic treatment (p?