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Data from experiment 1 were analysed as a randomized complete block design using PROC MIXED in SAS 9.1 (Cary, NC, USA). Data from experiment 2 were analysed as a mixed model, repeated measures randomized complete block design. Mean comparisons were done using linear contrasts in a two-way analysis of variance (ANOVA). The best-fit variance/covariance matrices for each variable were chosen using Akaike's information criterion [http://www.selleckchem.com/products/Y-27632.html this website] (Littell, Henry & Ammerman 1998; Littell, Pendergast & Natarajan 2000). At the end of experiment 1, the dry weight of NB in the control was similar to that of SL46 (Fig.?1a). However, there was a significant decrease in dry weight under ozone in both genotypes (34% for NB and 15% for SL46), and the ozone-by-genotype effect was significant (P?=?0.03). In the ozone treatment, [http://www.selleckchem.com/products/AZD2281(Olaparib).html www.selleckchem.com/products/AZD2281(Olaparib).html] the dry weight of SL46 was significantly higher than that of NB (Fig.?1a, P?=?0.013). Similar trends were observed in both the aboveground and belowground biomass (Fig.?1b,c); as a result, the root-to-shoot ratio was unchanged by ozone treatment in either genotype (Fig.?1d). By day 5 of the ozone fumigation in experiment 1, there was a significant decrease in midday carbon assimilation rate, as well as stomatal conductance in both genotypes (Tables?1 & 2). However, by the third week of ozone fumigation, SL46 showed significantly less reduction of carbon assimilation rate under ozone stress compared to NB (25 and 41%, [https://en.wikipedia.org/wiki/Bepotastine bepotastine] respectively; P?=?0.015; Table?1), but there was no genotypic difference in stomatal conductance (Table?2). At day 5 of the fumigation, there was no significant difference between NB and SL46 in any of the chlorophyll fluorescence parameters (Tables?2 & 3). However, by day 23, the ��PSII of NB was significantly decreased by ozone (?23%), whereas SL46 showed no significant difference between control and ozone treatments. Fv��/Fm�� showed a statistically significant decrease in SL46 under ozone, but the magnitude of the response was very similar between genotypes (?6.1 and ?8.7% for NB and SL46, respectively). However, the response of Fq��/Fv�� to ozone differed between the genotypes (Table?2). Fq��/Fv�� did not significantly change in SL46, but dropped by 17.6% in NB. At the third week of fumigation, SL46 showed significantly less reduction of biochemical photosynthetic capacity under ozone stress compared to NB (Fig.?2). The maximum carboxylation capacity of Rubisco (Vc,max) decreased by 24% in SL46 compared to 49% in NB (P?