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CBF values were calculated based on the value at Tmax of the tissue residual function (R(t)) derived using block-circulant singular value decomposition (bcSVD) (Wu et al., 2003). In each case, all structural, diffusion and perfusion images were aligned using SPM8 (Wellcome Department of Cognitive Neurology, UCL, UK). ASL and DSC perfusion images were further normalized into the Montreal Neurological Institute (MNI) template space using SPM8. Subsequently, segmentation of ASL and DSC perfusion images into major vascular territories was performed using an automated region-of-interest (ROI) analysis based on a published template of vascular territories in [http://www.selleck.co.jp/products/DAPT-GSI-IX.html DAPT in vivo] both hemispheres (Tatu et al., 1998). The vascular territories studied included leptomeningeal and lenticulostriate (perforator) MCA. In addition, the infarction core was defined as apparent diffusion coefficient value [http://www.selleck.co.jp/products/Gefitinib.html Selleck Gefitinib] between the two modalities. Pearson correlation coefficients were calculated [http://en.wikipedia.org/wiki/Histamine_H2_receptor Histamine H2 receptor] between asymmetry ratios of ASL and DSC for each of the perfusion parameters respectively. Pearson correlation coefficients were also calculated between asymmetry ratios of ASL and DSC perfusion parameters and NIH Stroke Scale (NIHSS) scores. Partial correlation analyses were carried out between ASL and DSC by including NIHSS and/or field strength as covariates. Paired t-test was applied for comparing quantitative CBF values in MCA territories and infarct ROIs derived using 4 PLDs and a single PLD of 2?s ( Qiu et al., 2012). The significance level was defined as p?��?0.05 (2-sided). Image data from 24 patients (mean age 79.7?��?11.4?years; 11 men) with acute MCA ischemic stroke were included. NIHSS scores ranged from 1 to 36, with a median of 16.5. The median time from stroke onset to imaging was 4.7?h (range 1.6 to 38.3?h). Half of the 24 patients were scanned on 1.5?T and another half on 3?T. Demographic and clinical information of the 24 stroke patients are provided in Supplemental Table?1. Table?1 lists the Pearson correlation coefficients between 4-delay pCASL and DSC asymmetry ratios of multiple perfusion parameters.