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1185 and P?=?0.0207) and (P?=?0.0038 and P?=?0.0001), respectively ( Fig. 3b). Images for two-dimensional scratch-migration assay were documented at fivefold magnification[17]. Transfection of miR-615-5p mimics led to significant reduction in tumor cell migration in HuH-7 and HepG2 cells covering 77.86% and 45.3% of the original scratch compared to mock and Scr-miR with original scratch coverage of (84.2%, 82.7%) and (100%, 100%) in both cell lines respectively. While miR-155 mimics resulted in promotion of tumor cell migration in HuH-7 and HepG2 cells covering 89% and 100% of the original scratch compared to mock and Scr-miR with original scratch coverage of (84.2%, 82.7%) and (100%, 100%) in both cell lines respectively (Fig. 4). Migration [http://en.wikipedia.org/wiki/17-Dimethylaminoethylamino-17-demethoxygeldanamycin 17-DMAG (Alvespimycin) HCl] inhibition by miR-615-5p was rescued using miR-615-5p anatgomirs in HuH-7 and HepG2 cells, while migration induction by miR-155 was repressed using miR-155 antagomirs (Fig. 4). miR-615-5p accession number and mature sequences were retrieved using miRBase database (http://www.mirbase.org/). In silico predictions was carried out using 4 different software, IGF-II was found to be [http://www.selleckchem.com/products/gsk2656157.html click here] a predicted target for miR-615-5p. miR-615-5p was found to hit IGF-II at 5 different regions by the same seed sequence (Fig. 5a). Binding scores given by softwares representing the hybridization energies exerted for miR-615-5p-IGF-II binding are shown in Fig. 5b. Mimicking of miR-615-5p in HuH-7 and HepG2 cells resulted in a significant downregulation of IGF-II mRNA levels (P?=?0.0001 and P?[http://www.selleckchem.com/products/i-bet151-gsk1210151a.html I-BET151] to miR-615-5p expression in the same patients. Using Pearson statistical method of correlation, miR-615-5p expression was found to be inversely correlated with IGF-II transcript expression in all HCC tissues studied with Pearson r?=??0.468 (P?=?0.0371, Fig. 7). To confirm that miR-615-5p directly targets the 3��UTR of IGF-II, 3��UTR target region was inserted downstream to luciferase reporter gene in pmiRGLO vector. Experiments were carried out by trasnfecting HuH-7 cells with either empty pmiRGLO vector or the vector with the miR-615-5p target region insert. miR-615-5p mimics and inhibitors were co-transfected with both vectors. Upon miR-615-5p mimics co-transfection, luciferase activity was inhibited by 19.4% in cells with the pmiRGLO?+?insert compared to cells transfected with the pmiRGLO�Cinsert (P?