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Further reduction incurs an extra cost and, thus, is not justifiable for enzymatic hydrolysis (Kim and Holtzapple, 2006). Therefore, a higher removal of lignin is not necessary for the enzymatic hydrolysis of the lignocellulosic biomass. The pulps obtained in this paper contain composition characteristics close to those recommended by Chang and Holtzapple (2000) for use in enzymatic hydrolysis, i.e., the absence of acetyl groups and a low lignin content ([https://en.wikipedia.org/wiki/Alizarin Alizarin] taking into account lignin removal are: (1) 198.3?��C, 150?min, and glycerol:water content of 80:20 and (2) 190?��C, 240?min, and glycerol:water content of 80:20. The choice of the best treatment condition related to energy requirement and time is the first option because an increase of 4.4% in the [http://www.selleckchem.com/products/Fulvestrant.html progestogen antagonist] reaction temperature results in a time economy of 90?min, which increases the amount of biomass processed. The organosolv process studied showed potential as method of delignification that still preserves the cellulose fraction. The best results obtained in the present study correspond to following pulp quality: residual lignin amount less than 8%; extent of delignification close to 80%; and residual cellulose content higher than 80%. The studies developed showed that pulping experiments with higher glycerol content liquors exhibited better results for amounts of delignification and residual cellulose than those using higher water content solutions. Furthermore, the obtained results demonstrated the possibility of applying crude glycerol directly from biodiesel production as delignification agent, making the process economically feasible. The authors are thankful to the University of S?o Paulo and PET group from the Chemistry Institute of S?o Carlos for providing a scholarship to BS L��sias Pereira Novo. The authors are also grateful to Conselho Nacional de Pesquisa e Desenvolvimento (CNPq) for providing a scholarship [http://www.selleckchem.com/products/SB-203580.html SB203580 in vitro] to PhD Leandro Vin��cius Alves Gurgel and for funding this research. ""In Table 1, page 3472, the ��F for compound 1 should be 0.012, not 0.12. The authors would like to apologise for any inconvenience caused. ""Surfactants are one of the most versatile products present in several industrial segments, such as manufacturing of motor oils, cleaning products and pesticides. Linear alkylbenzene sulfonate (LAS) is a widely used anionic surfactant due to its excellent properties and low cost (Garcia et al., 2005). Its wide use results in its presence in industrial wastewater and domestic sewage. As the consequence of its intense use and recalcitrance in wastewater, LAS has affected the efficiency of sewage treatment plants and has affected terrestrial and aquatic biota. LAS can inhibit self-purification processes in water courses and disseminate impurities.