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Among the treatments, the highest conversion (78.3%) was obtained for treatment 8 (10.2 ethanol:soybean oil molar [http://www.selleckchem.com/products/DAPT-GSI-IX.html DAPT secretase cost] ratio; 21% by oil wt. of enzyme; 80% of TLL in the mixture TLL/RML). The experimental data have been adjusted to the proposed model by the second-order polynomial Eq. (2), and the adequacy of the model was performed by analysis of variance and the parameters R and R2. The second-order polynomial model to transesterification reaction is presented in Eq. (3). equation(3) T=45.75+4.00X1?0.502X1+14.37X2+2.352X2+8.47X3+2.412X3?0.12X1X2?1.28X1X3?0.02X2X3T=45.75+4.00X1?0.50X12+14.37X2+2.35X22+8.47X3+2.41X32?0.12X1X2?1.28X1X3?0.02X2X3where T is the percentage of yield of conversion for transesterification reaction, and X1, X2, and X3, are the coded values of substrate molar ratio, enzyme content, and TLL/RML ratio, respectively. Statistical testing of the model was done by the Fisher's statistical test for analysis of variance (ANOVA). The computed F-value (37.09) was highly significant (p?[http://www.selleckchem.com/Proteasome.html click here] of the process model and a good correlation between the experimental results and the theoretical values predicted [https://en.wikipedia.org/wiki/Tolmetin Tolmetin] by the model equation. The linear, quadratic, and the interaction effects of the variables substrate molar ratio, enzyme content, and TLL/RML ratio are presented in Table 3. All linear effects were statistically significant and positive, which indicates that an increase in these variables should positively affect the yields of conversion, with the content of enzyme showing the highest effect. The positive effect of the mixture of TLL/RML suggests that higher amounts of TLL in the mixture will work to enhance the reaction. Although TLL is considered to be more efficient for transesterification, the results showed that a small addition of RML improves the yields of this conversion. This may be explained by the different specificity of each lipase for the diverse fatty acids presented in the soybean oil. Apparently, TLL is more active than RML over most of the tri-, di- and mono-glycerides present in the reaction with soybean oil, but RML could exhibit higher activities on some of them, producing a mixture of lipases much more efficient than the individual ones. For example, Lipozyme RM-IM displays high specificity towards linolenic acid [31], present in soybean oil [32].