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8?g protein); and pasteurized hen's egg (5?mg to 4.6?g protein). The severity of clinical symptoms was graded following a five-level grading system of food-induced anaphylaxis [22, 23]. However, we rated diarrhea as comparable in severity to repetitive vomiting (severity grade III, not IV). Grade I: skin symptoms: localized pruritus, flushing, urticaria, angioedema; gastrointestinal (GI) tract symptoms: oral pruritus, oral ��tingling,�� mild lip swelling; no respiratory, cardiovascular or neurological symptoms. Grade II: skin symptoms: generalized pruritus, flushing, urticaria, angioedema; GI tract symptoms: any of the above and/or nausea or a single episode of emesis; respiratory symptoms: nasal congestion and/or sneezing; no cardiovascular symptoms. Grade III: skin symptoms: any of the above; GI [http://en.wikipedia.org/wiki/Hesperadin Hesperadin] tract symptoms: any of the above plus repetitive vomiting or diarrhea; respiratory symptoms: rhinorrhea, marked congestion, sensation of throat pruritus or tightness; cardiovascular symptoms: tachycardia (increase: 15 beats/min). Grade IV: skin symptoms: any of the above; [http://www.selleckchem.com/products/ABT-737.html ABT-737 datasheet] GI tract: any of the above; respiratory symptoms: any of the above plus hoarseness, ��barky�� cough, difficulty swallowing, dyspnea, wheezing, cyanosis; cardiovascular symptoms: any of the above plus dysrhythmia and/or mild hypotension. Grade V: skin symptoms: any of the above; GI tract symptoms: any of the above, loss of bowl control; respiratory symptoms: any of the above, respiratory arrest; cardiovascular symptoms: severe bradycardia and/or hypotension or cardiac arrest, loss of consciousness. Blood was drawn prior to the FC. Patient serum was analyzed for specific IgE to cow's milk, hen's egg, wheat, and soy, using the Immuno-CAP-System FEIA? (Phadia, Uppsala, Sweden; lower detection limit 100?kU/l, the serum was diluted to acquire exact levels. For the statistical [http://www.selleckchem.com/products/torin-1.html mTOR inhibitor] analysis, we used SAS 9.2 (SAS Institute Inc, Cary, NC, USA). Only children with complete data for symptom severity and the last dose step were included. Logistic regression models were used for the prediction of challenge positivity, symptom severity (��grade II), and dose step (reaction before last dose). The models included adjustment for age (