Salebabies1: Створена сторінка: Due to the poor aqueous solubility of CQ and its copper complex Cu(CQ)2,[12b] analyses were performed in DMSO containing 10?vol?% [http://en.wikipedia.org/wiki/...

2017-01-25T02:58:03Z

Створена сторінка: Due to the poor aqueous solubility of CQ and its copper complex Cu(CQ)2,[12b] analyses were performed in DMSO containing 10?vol?% [http://en.wikipedia.org/wiki/...

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Due to the poor aqueous solubility of CQ and its copper complex Cu(CQ)2,[12b] analyses were performed in DMSO containing 10?vol?% [http://en.wikipedia.org/wiki/Cofactor_(biochemistry) Cofactor] of HEPES buffer, pH?7.4. When CQ was added to CuII�CA��1?16 (Cu�CA��/CQ=1:1 and Cu�CA��/CQ=1:2; Figure?5?b,c, respectively), the signal of CuII�CA��1�C16 disappeared and a series of resonances different from that of CuII(CQ)2 appeared with an A|| value of 193?G and a g|| value of 2.225, along with that of CuII(CQ)2 (Figure?5?d). This feature suggests that both CQ and A��1�C16 were acting as ligands of Cu2+. Figure 5 Electron spin resonance (ESR) spectra of a)?CuII�CA��1�C16, b)?CuII�CA��1�C16/CQ (1:1?mol ratio), c)?CuII�CA��1�C16/CQ (1:2?mol ratio), d)?Cu(CQ) ... The ternary complex A��1?16�CCu�CCQ was further characterized by mass spectrometry (MS). The Cu�CA��1?16 complex was first prepared; 1? mol?equiv of CQ was then added, and the mixture was immediately analyzed by MS using positive-mode electrospray ionization [http://www.selleckchem.com/products/Everolimus(RAD001).html selleck chemicals] (ESI+). Along with the peaks corresponding to A��1?16 (m/z=978.5, 652.6, 489.7, and 392.0 amu, for z=2, 3, 4, and 5, respectively), a series of multicharged peaks was detected at m/z 1161.9, 774.9, and 581.4 amu with z=2, 3 and 4, respectively (Figure?6?a). This pattern can be assigned to a ternary complex A��1�C16�CCu�CCQ with molecular formula C93H122ClCuIN28O29. The isotopic patterns were consistent with the theoretical profiles (Figure?6?b). The complex [http://www.selleckchem.com/products/PD-0332991.html Palbociclib mw] Cu�CA�� was also detected at m/z 1008.9, 672.9, 505.0, and 404.2 (z=2, 3, 4, and 5, respectively), indicating that a significant amount of Cu�CA�� was not affected by the presence of CQ (ca. 20?% with respect to free A��; for the full-scale spectrum, see Figure?S1 in the Supporting Information). Figure 6 Electrospray ionization mass spectrum (ESI+-MS) of CuII/A��1�C16/CQ (1:1:1?mol ratio). a)?Experimental spectrum and b)?the theoretical pattern for the ternary complex A�¨CCu�CCQ, with molecular ... Under the same conditions, in a mixture containing Cu�CA�� and ligand 1, signals of a putative complex A�¨CCu�C1 were not detected (Figure?S2 in the Supporting Information). The major detected compounds were free A�� and CuII�C1 (m/z=421.1 [M?H]+ and m/z=211.1 [M]2+). Only a tiny amount of Cu�CA�� was detected (

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Salebabies1: Створена сторінка: Due to the poor aqueous solubility of CQ and its copper complex Cu(CQ)2,[12b] analyses were performed in DMSO containing 10?vol?% [http://en.wikipedia.org/wiki/...