Knot32gallon: Створена сторінка: Sixteen mice that had not undergone treadmill training were divided into two groups of eight: saline and raclopride. Each mouse injected with saline or raclopri...

2017-03-03T09:53:13Z

Створена сторінка: Sixteen mice that had not undergone treadmill training were divided into two groups of eight: saline and raclopride. Each mouse injected with saline or raclopri...

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Sixteen mice that had not undergone treadmill training were divided into two groups of eight: saline and raclopride. Each mouse injected with saline or raclopride was acclimated to a whole-body single-chamber plethysmograph for ?30 min before exposure to gases. Gases were infused through [http://www.selleckchem.com/products/PLX-4720.html find more] a Plexiglass chamber placed on the plesythmograph chamber (PLY3211; Buxco Electronics). After exposure to 100% O2 for >5 min, the gas was carefully switched to 5% CO2 in O2, and then to 7 and 9% CO2 in O2 at 5 min intervals. Respiratory patterns during gas exposures were analysed for the mean value during 1 min at the steady state. Arterial blood gases were measured during resting conditions in saline-treated (n= 4) and raclopride-treated mice (n= 6). To sample blood, an arterial catheter was inserted into the right carotid artery and ligated with a surgical thread under general anaesthesia (pentobarbital sodium, 25 mg kg?1, i.p.) as described previously (Ishiguro et al. 2006). After surgery, each mouse was placed in a plastic chamber (90 mm �� 120 mm �� 50 mm), in which it was allowed to recover from anaesthesia for ��4 h. Initially, a mouse in the plastic chamber had its arterial blood collected. This was immediately [https://en.wikipedia.org/wiki/Reelin Reelin] analysed for pH, arterial CO2 partial pressure () and arterial O2 partial pressure () with a blood gas analyser (OPTI CCA; AVL Scientific, Roswell, GA, USA). After 30 min, the mouse was intraperitoneally administered saline or raclopride (2 mg (kg BW)?1). After [http://www.selleckchem.com/products/MS-275.html Entinostat cost] 20 min, the second collection was undertaken for the analyses of blood gases in the saline- or raclopride-treated mouse. Data are means �� SD. Data of respiration and pulmonary gas exchange in the resting state were averaged during a stable period 10 min before the start of treadmill movement, and analysed by Student's unpaired t test between treatments with saline and raclopride (Table 1). Data for blood gases were analysed by Student's unpaired t test (Table 2). Data for parameters of respiration and pulmonary gas exchange during treadmill running were analysed by two-way ANOVA. They were tested for effects of time (within-group factor used as a repeated measurement) and treatment (between-group factor) and for their interactions (Figs 2 and 4). Increases in fR and were analysed between saline and raclopride groups by Student's unpaired t test (Fig. 3). The relationship between and was examined by a regression analysis in each mouse group (Fig. 5). To compare the group effects of saline and raclopride, an ANCOVA was undertaken, with as a covariate. For these analyses, we used a commercially available software package (SPSS Japan, Inc., Tokyo, Japan). A value of P

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Knot32gallon: Створена сторінка: Sixteen mice that had not undergone treadmill training were divided into two groups of eight: saline and raclopride. Each mouse injected with saline or raclopri...