Yarn43angle: Створена сторінка: We investigated the regulation of IL-8 production in human melanocytes, and the IL-8 serum levels and skin gene expression in patients with vitiligo and in cont...

2017-03-28T02:02:50Z

Створена сторінка: We investigated the regulation of IL-8 production in human melanocytes, and the IL-8 serum levels and skin gene expression in patients with vitiligo and in cont...

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We investigated the regulation of IL-8 production in human melanocytes, and the IL-8 serum levels and skin gene expression in patients with vitiligo and in controls. Cultured melanocytes were stimulated for 24?h with tumour necrosis factor (TNF) 100?ng/mL and IL-1�� 10?ng/mL, with or without pretreatment with luteolin 50?��mol/L for 30?min, and IL-8 release was measured by ELISA. Serum cytokines were measured by a microbead array. Skin biopsies were taken from healthy subjects (n?=?14) as well as from marginal lesional and nonlesional skin from patients with vitiligo (n?=?15). IL-8 gene expression was evaluated by quantitative real time PCR. Both TNF and IL-1�� stimulated significant IL-8 release (P?[http://www.selleckchem.com/products/dorsomorphin-2hcl.html find more] pretreatment with luteolin significantly inhibited this effect [http://en.wikipedia.org/wiki/Resminostat Resminostat] (P?[http://www.selleckchem.com/products/Staurosporine.html selleck compound] IL-8 is chemotactic to neutrophils, T-lymphocytes, basophils and keratinocytes.[5] We studied the effect of TNF and IL-1�� on IL-8 release from human cultured primary melanocytes, before and after treatment with the natural flavonoid luteolin. We also studied the gene expression and serum levels of IL-6, IL-8 and VEGF in biopsies taken from controls and from the affected and nonlesional skin of patients with vitiligo. Human primary melanocytes from ATCC (PCS-200�C013) were cultured in cell medium [Dermal Basal Cell Medium; PCS-200-030; American Type Culture Collection (ATCCP) Manassas, VA, USA] supplemented with 100?U/mL penicillin/streptomycin (Life Technologies, Grand Island, NY, USA) and 50?��L phenol red (PCS-999�C001; ATCC). Incubation of melanocytes with TNF 100?ng/ml (210-TA-010; R & D Systems, Minneapolis, MN, USA) for 24?h induced significant (P?

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Yarn43angle: Створена сторінка: We investigated the regulation of IL-8 production in human melanocytes, and the IL-8 serum levels and skin gene expression in patients with vitiligo and in cont...