Відмінності між версіями «The Entire Study Behind E-64»

Матеріал з HistoryPedia
Перейти до: навігація, пошук
(Створена сторінка: In the laboratory, the inoculated swabs were immersed in 1?mL of brain-heart infusion and cultured onto a [https://en.wikipedia.org/wiki/E-64 E-64] selective va...)
 
м (The Entire Study Behind E-64)
 
Рядок 1: Рядок 1:
−
In the laboratory, the inoculated swabs were immersed in 1?mL of brain-heart infusion and cultured onto a [https://en.wikipedia.org/wiki/E-64 E-64] selective vancomycin-containing Columbia blood agar (2?mg/L). Plates were incubated aerobically at 37��C in a 5%CO2-enriched atmosphere, and examined once a day for two consecutive days. Kingella kingae was identified by its typical beta-haemolysis and biochemical characteristics, negative catalase and positive oxidase, and then confirmed by real-time PCR [1]. Every child had at the same time a synovial fluid sample obtained by arthotomy or arthroscopy during the surgical procedure and each sample was cultured in a blood culture vial of BacT/Alert 3D system (Biom��rieux, Craponne, France) as previously described [1]. The susceptibility to various antibiotics was determined as previously described [8]. In order to determine if K.?kingae pharyngeal isolates were the same strain as that responsible for the septic arthritis, we compared the sequences of the rtxA gene from the strains with that obtained from nucleic acid extracts of joint-fluids. Amplification of rtxA (1198?bp) was performed as previously described by Lehours et?al. [13]. Sequencing of the strands was realized by Beckman�CCoulter Genomics (Takeley, UK). The relatedness between each rtxA sequence was shown as a dendrogram, constructed by the Neighbour Joining method using the MEGA3.1 program. [http://www.selleckchem.com/products/jq1.html selleck products] To compare proportions we used either the chi-squared test or McNemar��s test when comparisons concern matched pairs of subjects. A p-value of [http://www.selleckchem.com/products/r428.html R428] Only 12 (11.9%) strains of K.?kingae were cultivated (11 in joint-fluid and one in blood culture). In these 12 cases, PCR was also positive in the synovial fluid. Real-time PCR was 7.4-fold more sensitive than the synovial or blood culture in our series (89 diagnoses vs. 12, respectively). Between March and September 2011, 13 children were hospitalized for documented K.?kingae arthritis. The mean age of these patients was 16?months (9�C47?months). One child had received cefpodoxime-proxetil for 1?day before throat sampling and was therefore excluded from the analysis. Kingella kingae isolates were cultivated in eight out of the 12 throat samples (66.7%) but in none of the 12 joint fluids. Consequently, with 66.7% positive cultures, throat culture was significantly more sensitive to cultivation of K.?kingae than joint-fluid or blood cultures in children with K.?kingae arthritis (66.7% vs. 0% in the prospective study, p?0.01, and 66.7% vs. 11.9% in the retrospective study, p?
+
A magnetic resonance imaging (MRI) probe was prepared by crosslinking ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles to the aptamer for tumor vascular endothelial growth factor 165 (VEGF165-aptamer). The molecular probe was evaluated for its in vitro and in vivo activities toward VEGF165. Enzyme-linked immunosorbent assay showed that the VEGF165-aptamer�CUSPIO nanoparticles conjugate specifically binds to VEGF165 in vitro. A cell proliferation test showed that VEGF165-aptamer�CUSPIO seems to block the proliferation of human umbilical vein endothelial [http://www.selleckchem.com/products/jq1.html JQ1] cells induced by free VEGF165, suggesting that VEGF165 is an effective target of this molecular probe. In xenograft mice carrying liver cancer that expresses VEGF165, T2-weighted imaging of the tumor displayed marked negative enhancement 3?h after the intravenous administration of VEGF165-aptamer�CUSPIO. The enhancement disappeared 6?h after administration of the probe. These results suggest the targeted imaging effect of VEGF165-aptamer�CUSPIO probe in vivo for VEGF165-expressing tumors. This is the first report of a targeted MRI molecular probe based on USPIO and VEGF165-aptamer. Copyright ? 2014 John Wiley & Sons, Ltd. ""63005" "The development of magnetic resonance imaging (MRI) contrast agents targeting [https://en.wikipedia.org/wiki/E-64 E-64] epitopes in atherosclerosis is of general interest. In particular, early detection of activated platelets as key players in plaque rupture could provide improved triage of patients. However, so far the efficiency of contrast agents targeting human pathologies can only be examined in animal experiments, which do not necessarily reflect human in vivo conditions. We therefore describe application of a contrast agent targeting activated human platelets in an MRI tissue flow chamber, allowing detection and characterization of contrast agent binding. Microparticles of iron oxide (MPIO) were conjugated to an antibody [http://www.selleckchem.com/products/r428.html R428 molecular weight] targeting ligand-induced binding sites (LIBS) on the activated platelet glycoprotein IIb/IIIa-receptor or to control antibody, resulting in LIBS�CMPIO or control�CMPIO contrast agent. Human endarterectomy specimens from patients with acute stroke or transient ischemic attack were imaged ex vivo before and after contrast agent perfusion using a 9.4?T MRI system. Specimens were measured under static (n?=?18) or flow conditions (n?=?18) in a specially designed flow chamber setup, simulating physiological conditions in a stenosed vessel. A significant MPIO-induced negative contrast was achieved in MRI by LIBS�CMPIO in specimens under static and flow conditions (LIBS�CMPIO vs control�CMPIO: p?

Поточна версія на 21:20, 7 червня 2017

A magnetic resonance imaging (MRI) probe was prepared by crosslinking ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles to the aptamer for tumor vascular endothelial growth factor 165 (VEGF165-aptamer). The molecular probe was evaluated for its in vitro and in vivo activities toward VEGF165. Enzyme-linked immunosorbent assay showed that the VEGF165-aptamer�CUSPIO nanoparticles conjugate specifically binds to VEGF165 in vitro. A cell proliferation test showed that VEGF165-aptamer�CUSPIO seems to block the proliferation of human umbilical vein endothelial JQ1 cells induced by free VEGF165, suggesting that VEGF165 is an effective target of this molecular probe. In xenograft mice carrying liver cancer that expresses VEGF165, T2-weighted imaging of the tumor displayed marked negative enhancement 3?h after the intravenous administration of VEGF165-aptamer�CUSPIO. The enhancement disappeared 6?h after administration of the probe. These results suggest the targeted imaging effect of VEGF165-aptamer�CUSPIO probe in vivo for VEGF165-expressing tumors. This is the first report of a targeted MRI molecular probe based on USPIO and VEGF165-aptamer. Copyright ? 2014 John Wiley & Sons, Ltd. ""63005" "The development of magnetic resonance imaging (MRI) contrast agents targeting E-64 epitopes in atherosclerosis is of general interest. In particular, early detection of activated platelets as key players in plaque rupture could provide improved triage of patients. However, so far the efficiency of contrast agents targeting human pathologies can only be examined in animal experiments, which do not necessarily reflect human in vivo conditions. We therefore describe application of a contrast agent targeting activated human platelets in an MRI tissue flow chamber, allowing detection and characterization of contrast agent binding. Microparticles of iron oxide (MPIO) were conjugated to an antibody R428 molecular weight targeting ligand-induced binding sites (LIBS) on the activated platelet glycoprotein IIb/IIIa-receptor or to control antibody, resulting in LIBS�CMPIO or control�CMPIO contrast agent. Human endarterectomy specimens from patients with acute stroke or transient ischemic attack were imaged ex vivo before and after contrast agent perfusion using a 9.4?T MRI system. Specimens were measured under static (n?=?18) or flow conditions (n?=?18) in a specially designed flow chamber setup, simulating physiological conditions in a stenosed vessel. A significant MPIO-induced negative contrast was achieved in MRI by LIBS�CMPIO in specimens under static and flow conditions (LIBS�CMPIO vs control�CMPIO: p?

Отримано з http://istoriya.soippo.edu.ua/index.php?title=The_Entire_Study_Behind_E-64&oldid=186404