Відмінності між версіями «Henle et al. have shown that PACAP does not change the number of dendrites and branches, but reduces elimination of newly formed dendrites and branches caused»
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| − | [http:// | + | Values represent imply SEM of sixty neurons from a few [http://lmslw.com/comment/html/?298819.html As a result, an environmental supply of an infection could be suspected with the possibility of a reservoir of B. pinnipedialis in the prey consumed by the seals] independent experiments. Scale bar, 20 m.K252a also inhibited axon length of control cultures. In contrast, K252a did not affect dendrite length (Fig. 5A, C).In the present study, we addressed the morphological effects of PACAP during early in vitro development of primary cultured hippocampal neurons, by comparing with BDNF. We found that PACAP increases neurite length, which is specifically due to increased axon, but not dendrite, length, and total and primary neurite number and soma size. These effects of PACAP were mostly comparable to BDNF. The PACAP antagonist PACAP68 completely blocked both the PACAP- and BDNF-induced increase in axon length, but not dendrite length, indicating that PACAP shows morphological actions via PAC1 receptors and that PACAP signaling might be involved in the BDNF-induced axon outgrowth. Previous studies in immortalized cell lines have indicated that PACAP has a uniform stimulating effect on various morphological features including total neurite length, total neurite number, extent of branching, and soma size [25,26]. In primary cultured neurons, lack of a Fig 5. The effect of TrkB receptor inhibitor K252a on neurite outgrowth. Primary hippocampal neurons were cultured with 10 nM PACAP or 2 nM BDNF in the presence or absence of 200 nM K252a for 3 DIV and double-immunostained for pNF and MAP2. Representative pNF- (red) and MAP2- (green) immunostained images of neurons (A), axon length (B), and dendrite length (C) were shown. Values represent mean SEM of 60 neurons from three independent experiments. P |
Поточна версія на 10:40, 1 грудня 2016
Values represent imply SEM of sixty neurons from a few As a result, an environmental supply of an infection could be suspected with the possibility of a reservoir of B. pinnipedialis in the prey consumed by the seals independent experiments. Scale bar, 20 m.K252a also inhibited axon length of control cultures. In contrast, K252a did not affect dendrite length (Fig. 5A, C).In the present study, we addressed the morphological effects of PACAP during early in vitro development of primary cultured hippocampal neurons, by comparing with BDNF. We found that PACAP increases neurite length, which is specifically due to increased axon, but not dendrite, length, and total and primary neurite number and soma size. These effects of PACAP were mostly comparable to BDNF. The PACAP antagonist PACAP68 completely blocked both the PACAP- and BDNF-induced increase in axon length, but not dendrite length, indicating that PACAP shows morphological actions via PAC1 receptors and that PACAP signaling might be involved in the BDNF-induced axon outgrowth. Previous studies in immortalized cell lines have indicated that PACAP has a uniform stimulating effect on various morphological features including total neurite length, total neurite number, extent of branching, and soma size [25,26]. In primary cultured neurons, lack of a Fig 5. The effect of TrkB receptor inhibitor K252a on neurite outgrowth. Primary hippocampal neurons were cultured with 10 nM PACAP or 2 nM BDNF in the presence or absence of 200 nM K252a for 3 DIV and double-immunostained for pNF and MAP2. Representative pNF- (red) and MAP2- (green) immunostained images of neurons (A), axon length (B), and dendrite length (C) were shown. Values represent mean SEM of 60 neurons from three independent experiments. P
