Відмінності між версіями «Noparticle tracking evaluation (NTA). To measure hypercoagulability and thrombin generation of»

Версія за 04:12, 27 грудня 2017

Kristinsson1 S about preferred procedures of collection in line with national preferences Division of Clinical Biochemistry, Aalborg University Hospital, Aalborg, Denmark; 2Department of Medicine, Division of Hematology, Karolinska University Hospital Solna, Stockholm, Sweden; 3Department of Hematology, University of Iceland and Landspitali National University Hospital, Reykjavik, IcelandIntroduction: Tissue element (TF), a transmembrane protein, is constitutively exposed on extravascular cells and their vesicles, and may be present in a non-coagulant plus a coagulant kind. DRM had been isolated by OptiPrep gradient ultracentrifugation, and 9 fractions of 1 ml were isolated and analysed for density, TF antigen (ELISA, western blot), TF coagulant activity (fibrin generation title= dar.12324 assay), flotillin (DRM marker) and caveolin (marker of DRM containing caveolae) and tissue element pathway inhibitor (TFPI; all western blot). Results: Vesicles of VSMC, wound bl.Noparticle tracking analysis (NTA). To measure hypercoagulability and thrombin generation of the patients, their plasma was analysed by indicates of calibrated automated thrombogram (CAT), and PPL activity was measured employing a chronometric process. TF levels were detected through an enzyme-linked immunoassay. Outcomes: Working with NTA, we observe improved particle levels in MGUS patients when compared to handle persons. Interestingly, sufferers exhibit considerably improved procoagulant activity and thrombin potential when when compared with handle persons. Also, the levels of TF in MGUS differ from that of handle persons, towards a extra diseased profile. Summary/conclusion: Within this potential study on MGUS sufferers, we found elevated levels of plasmatic EVs along with the hypercoagulable state in MGUS sufferers compared to matched controls. We strain the value of identifying the EV subpopulations that can be responsible or contribute for the procoagulant state observed in MGUS. These findings may assist clarify the underlying mechanisms for thrombosis in many myeloma that will hopefully cause prevention of thrombotic events at an earlier state.O8A-Tissue issue is associated with two kinds of detergent-resistant membranes in extracellular vesicles Anita N. Boing1, Chi M. Hau1, Jenny van den Goor2, Najat Hajji1, Auguste ?Sturk1 and Rienk Nieuwland1 Laboratory of Experimental Clinical Chemistry, Academic Health-related Center, Amsterdam, The Netherlands; 2Cardiothoracic Surgery, Academic Health-related Center, Amsterdam, The NetherlandsO8A-Procoagulant extracellular vesicles within the individuals with monoclonal gammopathy of undetermined significance and their impact on the thrombogenic profile Th er Nielsen1, Shona Pedersen1, Malin Hultcrantz2, S en R. Kristensen1 and Sigurdur Y. Kristinsson1 Division of Clinical Biochemistry, Aalborg University Hospital, Aalborg, Denmark; 2Department of Medicine, Division of Hematology, Karolinska University Hospital Solna, Stockholm, Sweden; 3Department of Hematology, University of Iceland and Landspitali National University Hospital, Reykjavik, IcelandIntroduction: Tissue factor (TF), a transmembrane protein, is constitutively exposed on extravascular cells and their vesicles, and can be present in a non-coagulant plus a coagulant kind. Within cell membranes, microdomains of detergent-resistant membranes (DRM) are present, that are enriched in cholesterol and sphingolipids when compared with their surroundings. DRM are resistant to non-ionic detergents, and cells include both low-density DRM (DRM-L r1.09?1.13 g/ml) and high-density DRM (DRM-H r1.15?.20 g/ml). We investigated title= per.1944 whether vesicles contain DRM, whether vesicleexposed TF is related with DRM and regardless of whether the association of TF with DRM could have an effect on the coagulant activity of TF. Solutions: Vesicles have been isolated from conditioned culture medium of humanCitation: Journal of Extracellular Vesicles 2014, 3: 24214 - http://dx.doi.org/10.3402/jev.v3.Scientific Plan 2014 ISEV meetingvascular smooth muscle cells (VSMC), human wound blood and human saliva. Vesicles had been lysed in Triton X-100-containing buffer.