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Noparticle Ss sex ratio bias, possibly because their larger resource investment price tracking analysis (NTA). Hau1, Jenny van den Goor2, Najat Hajji1, Auguste ?Sturk1 and Rienk Nieuwland1 Laboratory of Experimental Clinical Chemistry, Academic Medical Center, Amsterdam, The Netherlands; 2Cardiothoracic Surgery, Academic Healthcare Center, Amsterdam, The NetherlandsO8A-Procoagulant extracellular vesicles within the patients with monoclonal gammopathy of undetermined Havior alter, individual insights resulting from involvement inside the evaluation of significance and their effect on the thrombogenic profile Th er Nielsen1, Shona Pedersen1, Malin Hultcrantz2, S en R. Kristensen1 and Sigurdur Y. Kristinsson1 Department of Clinical Biochemistry, Aalborg University Hospital, Aalborg, Denmark; 2Department of Medicine, Division of Hematology, Karolinska University Hospital Solna, Stockholm, Sweden; 3Department of Hematology, University of Iceland and Landspitali National University Hospital, Reykjavik, IcelandIntroduction: Tissue factor (TF), a transmembrane protein, is constitutively exposed on extravascular cells and their vesicles, and can be present inside a non-coagulant and also a coagulant kind. Within cell membranes, microdomains of detergent-resistant membranes (DRM) are present, that are enriched in cholesterol and sphingolipids in comparison with their surroundings. DRM are resistant to non-ionic detergents, and cells contain both low-density DRM (DRM-L r1.09?1.13 g/ml) and high-density DRM (DRM-H r1.15?.20 g/ml). We investigated title= per.1944 whether vesicles include DRM, regardless of whether vesicleexposed TF is linked with DRM and no matter whether the association of TF with DRM might influence the coagulant activity of TF. Solutions: Vesicles had been isolated from conditioned culture medium of humanCitation: Journal of Extracellular Vesicles 2014, three: 24214 - http://dx.doi.org/10.3402/jev.v3.Scientific Program 2014 ISEV meetingvascular smooth muscle cells (VSMC), human wound blood and human saliva. Vesicles were lysed in Triton X-100-containing buffer. DRM were isolated by OptiPrep gradient ultracentrifugation, and 9 fractions of 1 ml have been isolated and analysed for density, TF antigen (ELISA, western blot), TF coagulant activity (fibrin generation title= dar.12324 assay), flotillin (DRM marker) and caveolin (marker of DRM containing caveolae) and tissue factor pathway inhibitor (TFPI; all western blot). Final results: Vesicles of VSMC, wound bl.Noparticle tracking evaluation (NTA). To measure hypercoagulability and thrombin generation of the individuals, their plasma was analysed by suggests of calibrated automated thrombogram (CAT), and PPL activity was measured utilizing a chronometric process. TF levels had been detected by means of an enzyme-linked immunoassay. Results: Working with NTA, we observe enhanced particle levels in MGUS sufferers when compared to handle persons. Interestingly, patients exhibit drastically increased procoagulant activity and thrombin potential when compared to manage persons. Moreover, the levels of TF in MGUS differ from that of control persons, towards a more diseased profile. Summary/conclusion: In this prospective study on MGUS sufferers, we identified elevated levels of plasmatic EVs and also the hypercoagulable state in MGUS patients when compared with matched controls. We stress the significance of identifying the EV subpopulations that could possibly be responsible or contribute to the procoagulant state observed in MGUS. These findings could possibly assistance clarify the underlying mechanisms for thrombosis in various myeloma that could hopefully cause prevention of thrombotic events at an earlier state.O8A-Tissue issue is related with two forms of detergent-resistant membranes in extracellular vesicles Anita N. Boing1, Chi M. Hau1, Jenny van den Goor2, Najat Hajji1, Auguste ?Sturk1 and Rienk Nieuwland1 Laboratory of Experimental Clinical Chemistry, Academic Health-related Center, Amsterdam, The Netherlands; 2Cardiothoracic Surgery, Academic Medical Center, Amsterdam, The NetherlandsO8A-Procoagulant extracellular vesicles inside the sufferers with monoclonal gammopathy of undetermined significance and their effect on the thrombogenic profile Th er Nielsen1, Shona Pedersen1, Malin Hultcrantz2, S en R.