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As a result the invaginating contacts outnumbered the ribbonsFrontiers in Neuroanatomy | www.frontiersin.orgOctober 2016 | Volume 10 | ArticleTsukamoto and OmiMacaque ON Bipolar Cell ConnectivityCells with live bacteria at 100 MOI in 12 effectively plates as above Figure five | Abundance in the 3 positional classes of synaptic contacts over the complete cone location for each and every ON bipolar cell form. (C) Pie charts displaying the typical proportions on the three positional classes of synapses. Data are mean ?SD for each bipolar cell type (variety of cells): IMB (six), BB (7), DB4 (six), DB5 (7), DB6 (5), GB (two) with SD (A-C). (D) A comparison of M/L-cone (left-handed bars, n = 12) and S-cone (right-handed bars, n = six) divergence for the six forms of ON bipolar cells. The abscissa is the average variety of contacts of a cone with every form of bipolar cells with all the SD bar. (E) Pie charts comparing M/L-cones and S-cones in the innervation of different bipolar forms. (F) Three positional classes of contacts: invaginationg, TA, and NTA in the ON and OFF routes diverging from an M/L-cone pedicle (P41) and an S-cone pedicle (P39) each located within the center of the examination area (Figure five).Frontiers in Neuroanatomy | www.frontiersin.orgOctober 2016 | Volume ten | ArticleTsukamoto and OmiMacaque ON Bipolar Cell ConnectivityTABLE 1 | Synaptic convergence of cones onto each ON bipolar cell sort. Bipolar cell variety (n) Number of cells Variety of cones per bipolar All Invagi. IMB (12) BB (7) DB4 (6) DB5 (7) DB6 (five) GB (two) 1.2 ?0.4 1.7 ?0.8 eight.7 ?1.0 7.3 ?1.1 6.two ?1.3 12.five ?0.7 26.three ?two.five 24.1 ?four.8 39.7 ?four.1 26.7 ?1.six 14.six ?two.9 16.five ?2.1 26.0 ?two.6 23.9 ?5.0 19.0 ?three.three 16.7 ?five.8 5.0 ?2.8 3.0 ?1.4 Variety of contacts per bipolar Positional classes TA 0.3 ?0.six 0.three ?0.five 16.7 ?2.6 7.1 ?4.3 eight.four ?two.7 six.0 ?1.4 NTA 0.0 ?0.0 0.0 ?0.0 4.0 ?two.8 2.7 ?two.9 1.two title= s12687-015-0238-0 ?0.8 7.5 ?0.7 Chromatic classes M/L-cone title= acr.22433 26.5 ?three.1 0.0 ?0.0 39.5 ?four.two 26.1 ?2.1 14.2 ?2.4 15.5 ?three.5 S-cone 0.0 ?0.0 24.1 ?4.8 0.two ?0.four 0.six ?1.5 0.four ?0.five 1 ?1.TABLE two | Synaptic divergence of M/L- or S-cones to distinctive ON bipolar cell kinds.Edicle P39 had 34 ribbons. Each and every variety of ribbons is close to its imply worth title= s13578-015-0060-8 at three mm eccentricity (Imply ?SD: 31.3 ?3.4 M/L-cone, n = 23; 31.8 ?two.eight for S-cone, n = ten, Tsukamoto and Omi, 2015a). 1 IMB and 7 DB cells had a total of 39 invaginating contacts with P41, whereas 4 BB cells had 43 invaginating contacts with P39. Thus the invaginating contacts outnumbered the ribbonsFrontiers in Neuroanatomy | www.frontiersin.orgOctober 2016 | Volume ten | ArticleTsukamoto and OmiMacaque ON Bipolar Cell ConnectivityFIGURE 5 | Abundance of the three positional classes of synaptic contacts over the entire cone area for every single ON bipolar cell variety.