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The total cellular ATP level was measured by an ATP bioluminescence detection assayThe intracellular ROS level was determined by a DCFH-DA fluorescence ROS detection assay
The total sub-G1 population is gated (pink box) and anything above roughly 103 should be positive for annexin V
The transcript levels of Hey1, Hey2 and ID2 were determined 1.5h after changing the medium and were normalized to the samples
The transfected cells ended up instantly transferred into 10 ml of SDM-seventy nine supplemented with G418 and hygromycin
The transfections had been done making use of LipofectamineTM LTX and PLUSTM reagents (Invitrogen)
The transfer of mycolic acids from trehalose monomycolate to trehalose dimycolate is catalyzed
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The trials addressed main immunization techniques with two successive vaccine doses
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The two cysteine residues of the 5 amino acid residue array at each of the two response centres
The two reaction centres belongs to one particular of 10 recognized types of plant proteinase inhibitors
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The uncoated upper side of each filter was wiped with a cotton swab to remove cells that had not migrated through the filter
The underlying body systems on which capability depended. Social and psychological
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The upregulation of HSP60 and PDI by azacytidine shows that the endoplasmic reticulum and mitochondria are the major targets of azacytidine-induced cell stress
The upstream peak was centred on the upstream margin of the cuff and the downstream peak was centred about .five mm downstream of the cuff
The uptake was terminated by the removal of the substratecontaining buffer, and the wells were washed three times with icecold HBSS
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Then the highest intensity between 05 mm into the wall (z-route) from the luminal area was obtained at each and every x-y location
Then the system was simulated in the NVT ensemble for Stabilization of whole vitality was attained in the very last
Then this cell lysate have been centrifuged six min at seven hundred g (4) to take away the nuclei
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Ther than for the other sequences (Figure 4). All C. parvum sequences
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