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The total cellular ATP level was measured by an ATP bioluminescence detection assayThe intracellular ROS level was determined by a DCFH-DA fluorescence ROS detection assay
The total sub-G1 population is gated (pink box) and anything above roughly 103 should be positive for annexin V
The transcript levels of Hey1, Hey2 and ID2 were determined 1.5h after changing the medium and were normalized to the samples
The transfected cells ended up instantly transferred into 10 ml of SDM-seventy nine supplemented with G418 and hygromycin
The transfections had been done making use of LipofectamineTM LTX and PLUSTM reagents (Invitrogen)
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The trials addressed main immunization techniques with two successive vaccine doses
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The two reaction centres belongs to one particular of 10 recognized types of plant proteinase inhibitors
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The uptake was terminated by the removal of the substratecontaining buffer, and the wells were washed three times with icecold HBSS
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Ther than for the other sequences (Figure 4). All C. parvum sequences
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