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- The PCR items from genomic DNA and cDNA had been subject to further sequencing evaluation for final verification
- The PCR items from genomic DNA and cDNA have been subject to further sequencing evaluation for final verification
- The PCR merchandise from genomic DNA and cDNA have been topic to additional sequencing analysis for final verification
- The PCR merchandise from genomic DNA and cDNA were subject to further sequencing analysis for final verification
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- The PCR protocol consisted of an original stage of five min at 94uC, followed by 35 cycles of 1 min at 94uC, one min at 50uC, and one:30 min at 68uC
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- The PDEs themselves did not have any impact on the endogenous fluorescence of MANT-substituted nucleotides, which is imperative for this approach
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