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- The relative microRNA and mRNA levels were computed using the 22delta delta Ct analysis method, where U6 and b-actin were used as internal controls, respectively
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- The response combination was stirred for 1 h at space temperature, and then evaporated underneath reduced strain
- The response mixtures were loaded onto the TLC plate and produced as described above
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- The results indicated that compared with untreated K562 cells, Icaritin-treated K562 cells (both 8 mM and 32 mM) had no significant effect on the expression of Bcr/Abl mRNA
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