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  3. The three cell lines studied showed greater E-NPP activity in alkaline pH, the optimum pH for these enzymes in vitro
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  27. The total cellular ATP level was measured by an ATP bioluminescence detection assayThe intracellular ROS level was determined by a DCFH-DA fluorescence ROS detection assay
  28. The total sub-G1 population is gated (pink box) and anything above roughly 103 should be positive for annexin V
  29. The transcript levels of Hey1, Hey2 and ID2 were determined 1.5h after changing the medium and were normalized to the samples
  30. The transfected cells ended up instantly transferred into 10 ml of SDM-seventy nine supplemented with G418 and hygromycin
  31. The transfections had been done making use of LipofectamineTM LTX and PLUSTM reagents (Invitrogen)
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  45. The two cysteine residues of the 5 amino acid residue array at each of the two response centres
  46. The two reaction centres belongs to one particular of 10 recognized types of plant proteinase inhibitors
  47. The two studies was tested working with a two-group model (see Table
  48. The type I insulin-like growth factor receptor (IGF-IR) is known to promote metastasis in several cancers
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  50. The uncoated upper side of each filter was wiped with a cotton swab to remove cells that had not migrated through the filter

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