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- Total RNA was extracted using a modified version of the TRIzol method (Invitrogen), in which RNA is purified directly from the aqueous phase using the Rneasy MinElute RNA purification kit
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- Total RNA was isolated using the TRIzol reagent (Invitrogen, Carlsbad CA, USA) according to the manufacturer's instructions
- Total RNA was reverse transcribed with oligo primer employing the M-MLV reverse transcriptase for RTPCR
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