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  23. Total RNA was extracted from embryos making use of ISOGEN and prepared for cDNA synthesis employing SuperScriptHII transcriptase
  24. Total RNA was extracted from embryos working with ISOGEN and ready for cDNA synthesis utilizing SuperScriptHII transcriptase
  25. Total RNA was extracted from neuroblasts using TRIzol reagent according to the manufacturer's instructions
  26. Total RNA was extracted using a modified version of the TRIzol method (Invitrogen), in which RNA is purified directly from the aqueous phase using the Rneasy MinElute RNA purification kit
  27. Total RNA was isolated from each C. elegans sample using a combination of TRIZOLH (Invitrogen, Karlsruhe, Germany) till the ethanol precipitation step followed by purification via the RNeasy Mini Kit
  28. Total RNA was isolated using the TRIzol reagent (Invitrogen, Carlsbad CA, USA) according to the manufacturer's instructions
  29. Total RNA was reverse transcribed with oligo primer employing the M-MLV reverse transcriptase for RTPCR
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  31. Total cell lysates were resolved by SDS-PAGE and immunoblotted with antibodies against PDCD4 or tubulin
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  33. Total protein was quantified by the Bio-Rad protein assay, and processed for Western blot using primary goat antibodies for VCAM-1 and ICAM-1
  34. Total scores of 3 were defined as positive to simplify data analysis. To analyze the correlation between CEACAM6 and E-cadherin expression
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