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- Cells were being lysed in RIPA buffer, and proteins were being isolated from mobile lysates by immunoprecipitation as explained earlier
- Cells were pelleted and lysed in 100 mL of DMSO, and the absorbance at 550 nm was measured using a microplate reader
- Cells were placed in transwells and allowed to migrate for 4 h in the presence of vascular endothelial growth factor
- Cells were stimulated with 10 ng/ml hTNF-a with or without twenty five mg/ml crude extract or still left untreated as damaging control and stained with mouse anti-human ICAM-one mAB
- Cells were then treated with or devoid of PEITC
- Cells were voltage-clamped in the conventional whole-cell configuration using an Axopatch 200B amplifier
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