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- To determine if pRb also plays a role in the NET4/Tmem53-directed effects, flow cytometry cell cycle/DNA content profiles were determined for cells expressing NET4/Tmem53 or controls in HEK293T cells with normal or reduced levels of pRb (06:48, 30 грудня 2016)
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- At 48 or 72 h coverslips were removed and fixed for immunocytochemistry and the remaining cells on the plate were harvested by trypsinization and lysed in sample buffer for SDSPAGE and Western blotting (07:00, 30 грудня 2016)
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