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- Fluorescence measurements were performed in a TECAN Microplate Reader (Model Infinite M200 Pro) with excitation and emission at 485 nm and 520 nm
- Fluorescence microscopy images were taken 24 hrs post-transfection to visualize the YFP fusion partner on each TFB and the images shown are representative of 3 separate experiments
- Fluorescence microscopy was used to identify and enumerate the translocation-positive cells at different times after infection
- Fluorescence spectra were attained using an excitation wavelength of 288 nm, with an excitation bandwidth of five nm
- Fluorescent images for a minimum of 50 nuclei had been captured utilizing an Olympus microscope and analyzed by CASP1
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