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- Tissue extracts were analysed in duplicate for CICP and MMP-1 using the same assays as culture supernatants, and IL-1b and TIMP-1 were analysed using R (2 версії)
- To Join The Web Design Big Leagues, You Need These Tips And Tricks (2 версії)
- To date, a model plant, Arabidopsis thaliana, has been subjected to thousands of microarray experiments and the results have been deposited in publicly-available online databases (2 версії)
- To determine gene expression, forward and reverse primers were designed (listed in Table 1) and used in RT-PCR reactions (2 версії)
- To determine if L-NAME treatment modified the ability of VEGF to activate this pathway, the phosphorylation state of eNOS and AKT after a 5-min VEGF stimulation in control and chronically L-NAME treated cells was measured (2 версії)
- To determine the number of replicates required to detect significant changes in fitness parameters resulting from silencing a defense gene (2 версії)
- To enable a simplified handling and precise positioning of zebrafish larvae and to achieve consistent visualization of tissues in high content screening scenarios (2 версії)
- To examine this possibility, we photobleached ABCB11-YFP in the entire canaliculus, and found that the rapid phase of fluorescence recovery was then virtually absent (2 версії)
- To explore the role of Not4p phosphorylation under these stress conditions, the not4S/T5A strain was subjected to 37uC, hydroxyurea or hygromycin B growth conditions (2 версії)
- To further confirm the critical role of ERK1/2 activation in p53 phosphorylation and HCV suppression by ribavirin, we performed an ERK1/2 knockdown experiment (2 версії)
- To further examine the molecular mechanisms regulating activity-dependent ST8SiaII expression, we turned to cortical organotypic cultures (2 версії)
- To further explore the temporal expression of the 15 miRNAs validated above in the developing embryonic breast muscle of Pekin duck (2 версії)
- To further this observation, we measured global gene expression patterns with microarray analysis by comparing total RNAs isolated from four-month-old contro (2 версії)
- To identify an early nonsurgical treatment for fibromatosis nodules before they progress to the stage where only surgical excision can be applied (2 версії)
- To induce TIME cell differentiation and the formation of tubular-like structures, ice-cold 12-well plates were coated with 200 ml growth factor-reduced Matrigel (2 версії)
- To induce differentiation, confluent preadipocytes(this day was marked as D0, the second day was marked as D1, and so on) were treated for 2 days with 0.5 mmol/L isobutylmethylxanthine (2 версії)
- To investigate the role of regulation in the response to periods of starvation and the subsequent metabolic recovery in more detail (2 версії)
- To more specifically investigate the relevant type 1-dependent functions of the SurA variant strains, we next assayed the binding and invasion of cultured human bladder epithelial cells by UPEC expressing the SurA variants (2 версії)
- To optimize the antifibrotic effects of this compound, we synthesised a series of cinnamoyl anthranilate derivatives of tranilast, based initially on their ability to inhibit TGF-induced collagen production (2 версії)
- To prepare His-tagged cofilin (cofilin-(His)6) construct, cDNA of cofilin was cloned into the BamH1 and NcoI sites of the pQE60 vector (2 версії)
- To provide additional evidence supporting the MS-based protein quantitation results, western blots were performed on selected proteins (2 версії)
- To recognize the biological processes represented by the genes (2 версії)
- To test our hypothesis, phosphorylation levels of EGFR and AKT had been examined in tumor lysates by western blotting (2 версії)
- To test our hypothesis, phosphorylation levels of EGFR and AKT have been examined in tumor lysates by western blotting (2 версії)
- To test this, we expressed PON2 and GFP, or GFP alone as control by adenoviral infection in DJ-1 WT or KO cortical neurons (2 версії)
- To this end, one must assume that the within-compound association between A and6mediates an initial excitatory relationship between6and the outcome (2 версії)
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