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- Human GnT-V (NM 002410.3) was cloned into lentiviral vector pCDH-puro. GnT-V overexpressed lentivirus was obtained by collecting supernatant of 293T after cotransfection with envelope
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- Human airway basal cells were infected with lentivirus expressing GFP (Lenti-GFP) or NICD1-4 (Lenti-NICD1-4) and cultured on ALI for 28 days
- Human airway basal cells were infected with lentivirus expressing GFP alone, NICD1, NICD2, NICD3 or NICD4 and cultured on ALI for 28 days
- Human alveolar macrophages are unique in this aspect since relatively pure populations can be obtained from bronchoalveolar lavage fluid
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- Human embryonic kidney (HEK) 293T cells were used for luciferase assay and maintained in DMEM supplemented
- Human embryonic stem cells are considered to be extremely metabolically energetic with a extremely glycolytic character
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- Human subject research approval for this study was received from the Western Institutional Review Board
- Human umbilical vein endothelial cells (HUVECs) were isolated from freshly derived umbilical cords by digestion with collagenase as described by Jaffe
- Humans specific two glutaminase isoforms: kidney-kind glutaminase and liver-kind glutaminase from two carefully linked genes
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