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  11. The protein concentration was then quantified using the Bradford Assay and an appropriate volume of 4X NuPAGE LDS sample buffer (Invitrogen) containing 200 mM dithiothreitol (DTT) added to each sample‏‎ (2 версії)
  12. The purposeful protein wants two such non-equivalent domains to fold with each other to type the disulphide bonds‏‎ (2 версії)
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  16. The raw data from the QSTAR ELITE were analyzed with MASCOT Daemon software (version 2.2.2, Matrix Science) using a local Mascot server‏‎ (2 версії)
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  20. The relative microRNA and mRNA levels were computed using the 22delta delta Ct analysis method, where U6 and b-actin were used as internal controls, respectively‏‎ (2 версії)
  21. The relative starting quantity for each experimental sample was calculated based on the standard curve made for each primer pair‏‎ (2 версії)
  22. The released and activated COOH-terminal fragment of Notch intracellular domain (NICD) translocates to the nucleus‏‎ (2 версії)
  23. The remaining pellet was resuspended in membrane extraction buffer containing protease inhibitors, incubated at 4uC for 10 minutes with gentle mixing‏‎ (2 версії)
  24. The results from the Luciferase experiments were entered to the MATLAB software and transformed using the fft algorithm‏‎ (2 версії)
  25. The results presented above indicate that measuring the killing rate profile of candidate antimalarials is a valid approach to identify fast acting compounds‏‎ (2 версії)
  26. The sacrifice of animals resulted from the removal of heart and lungs after the measurement of pulmonary arterial pressure‏‎ (2 версії)
  27. The sequence-specific oligonucleotide primers were selected with the use of the "Primer Express" software‏‎ (2 версії)
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  31. The specificity of the antisera was evaluated by ELISAs with the nonphosphorylated and phosphorylated antigenic peptides as well as by Western blot analysis using purified non-phosphorylated and autophosphorylated cGKI isozymes‏‎ (2 версії)
  32. The steady-state solutions of the biochemical variables for the PE Alternating Cycle correspond to the following expressions‏‎ (2 версії)
  33. The study was approved by the Institutional Review Board of the Second Xiangya hospital, central South University‏‎ (2 версії)
  34. The system process is defined as a multicellular organismal process carried out by any of the organs or tissues in an organ system‏‎ (2 версії)
  35. The total sub-G1 population is gated (pink box) and anything above roughly 103 should be positive for annexin V‏‎ (2 версії)
  36. The transcript levels of Hey1, Hey2 and ID2 were determined 1.5h after changing the medium and were normalized to the samples‏‎ (2 версії)
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  44. There appeared to be more variation in the relative degree of co-localization and differential expression of occludin and claudin-4 on the intact lateral membranes compared to what was observed during the day‏‎ (2 версії)
  45. There are two main K channels we studied in this content: the non-specific ATP-sensitive K channel and the Ca2 -activated K channel‏‎ (2 версії)
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  47. There is an inverse relationship between KD and affinity. Therefore, the binding affinity of mAb to EGFR was higher than that of EGF to EGFR‏‎ (2 версії)
  48. There is some evidence that intracellular PEDF may lack the secretion signal either through posttranslational processing or via mRNA splice variants‏‎ (2 версії)
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  50. There was no difference in the number of migrated cells in response to human SCF under all conditions analyzed‏‎ (2 версії)

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