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- The protein concentration was then quantified using the Bradford Assay and an appropriate volume of 4X NuPAGE LDS sample buffer (Invitrogen) containing 200 mM dithiothreitol (DTT) added to each sample (2 версії)
- The purposeful protein wants two such non-equivalent domains to fold with each other to type the disulphide bonds (2 версії)
- The quantity of hours worked per week and also the number of (2 версії)
- The quantity of hours worked per week and also the variety of (2 версії)
- The quantity of hours worked per week as well as the variety of (2 версії)
- The raw data from the QSTAR ELITE were analyzed with MASCOT Daemon software (version 2.2.2, Matrix Science) using a local Mascot server (2 версії)
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- The relative microRNA and mRNA levels were computed using the 22delta delta Ct analysis method, where U6 and b-actin were used as internal controls, respectively (2 версії)
- The relative starting quantity for each experimental sample was calculated based on the standard curve made for each primer pair (2 версії)
- The released and activated COOH-terminal fragment of Notch intracellular domain (NICD) translocates to the nucleus (2 версії)
- The remaining pellet was resuspended in membrane extraction buffer containing protease inhibitors, incubated at 4uC for 10 minutes with gentle mixing (2 версії)
- The results from the Luciferase experiments were entered to the MATLAB software and transformed using the fft algorithm (2 версії)
- The results presented above indicate that measuring the killing rate profile of candidate antimalarials is a valid approach to identify fast acting compounds (2 версії)
- The sacrifice of animals resulted from the removal of heart and lungs after the measurement of pulmonary arterial pressure (2 версії)
- The sequence-specific oligonucleotide primers were selected with the use of the "Primer Express" software (2 версії)
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- The specificity of the antisera was evaluated by ELISAs with the nonphosphorylated and phosphorylated antigenic peptides as well as by Western blot analysis using purified non-phosphorylated and autophosphorylated cGKI isozymes (2 версії)
- The steady-state solutions of the biochemical variables for the PE Alternating Cycle correspond to the following expressions (2 версії)
- The study was approved by the Institutional Review Board of the Second Xiangya hospital, central South University (2 версії)
- The system process is defined as a multicellular organismal process carried out by any of the organs or tissues in an organ system (2 версії)
- The total sub-G1 population is gated (pink box) and anything above roughly 103 should be positive for annexin V (2 версії)
- The transcript levels of Hey1, Hey2 and ID2 were determined 1.5h after changing the medium and were normalized to the samples (2 версії)
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- There appeared to be more variation in the relative degree of co-localization and differential expression of occludin and claudin-4 on the intact lateral membranes compared to what was observed during the day (2 версії)
- There are two main K channels we studied in this content: the non-specific ATP-sensitive K channel and the Ca2 -activated K channel (2 версії)
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- There is an inverse relationship between KD and affinity. Therefore, the binding affinity of mAb to EGFR was higher than that of EGF to EGFR (2 версії)
- There is some evidence that intracellular PEDF may lack the secretion signal either through posttranslational processing or via mRNA splice variants (2 версії)
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- There was no difference in the number of migrated cells in response to human SCF under all conditions analyzed (2 версії)
- Thereby, it would not be surprising if cancer cells decrease BAD expression (2 версії)
- Therefore, a third intracellular compartment, into which the drug permeates from the systemic circulation, is included (2 версії)
- Therefore, in this study, we investigated the cytotoxic potential of fatty acid esters of phloridzin on cell proliferation of solid tumours such as hepatocellular carcinoma HepG2 cells (2 версії)
- Therefore, studying formation and function of the zebrafish pronephros can aid in the understanding of the role of genes mutated in kidney disease (2 версії)
- Therefore, the identification of highly effective breast cancer therapies that do not negatively impact the skeleton, or that actually exhibit beneficial effects on bone health (2 версії)
- Therefore, we hypothesized that MeHg inhibits Hsp90-client protein interactions and consequently disrupts Hsp90 client protein functions (2 версії)
- Therefore, we measured IL-1b production in human CF macrophages after autophagy stimulation as a primary source of excess inflammatory cytokine production during infection (2 версії)
- Therefore, we restrict the sampling to an evolved metabolic state, such that all sampled parameters are consistent with this experimentally observed state (2 версії)
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- Therefore an opportunity for all of the groups to operate together for (2 версії)
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