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- Protein concentration was determined using the Bradford protein assay reagent (Bio-Rad) with bovine serum albumin (Promega) as standard
- Protein gels were stained with Coomassie Brilliant Blue R-250 (Sigma-Aldrich), and protein bands were excised from the gels, reduced, alkylated, and digested in-gel with trypsin
- Protein translocation is detected within the first hour of infection when BlaM-effector fusions are produced in Legionella
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- Proteinntibody complexes were visualized using the enhanced chemiluminescence Western blotting detection system according to the manufacturer's protocol
- Proteins launched in the blood-stream replicate the complicated community of features performing inside of the tissues
- Proteins with already established roles in DNA repair or cell cycle control were excluded, as were ribosomal proteins
- Proto-Oncogene Tyrosine-Protein Kinase Lck
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- Protocol allows such measurements without using pharmacological blockers of ionotropic glutamate receptors,leaving the possibility to use the same MSN for other pharmacological tests
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