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- Samples have been organized for Western blot examination in the existence of a protease inhibitor cocktail, which inhibits the activity of the proteasome, calpains and caspases but not that of lysosomal enzymes
- Samples shown to be b-globin positive were then examined for bacterial quantification by Real time PCR
- Samples were subjected to immunoblotting using a monoclonal GST, a monoclonal PEDF and monoclonal Transportin-SR2 antibody
- Samples were then transferred to an airtight chamber maintained at 30uC, and the oxygen content was monitored for at least 5 min
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