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  9. Samples from Caucasian subjects have been added to evaluate irrespective of whether similar patterns of DNA methylation and mRNA expression had been observed
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  19. Samples have been organized for Western blot examination in the existence of a protease inhibitor cocktail, which inhibits the activity of the proteasome, calpains and caspases but not that of lysosomal enzymes
  20. Samples shown to be b-globin positive were then examined for bacterial quantification by Real time PCR
  21. Samples were subjected to immunoblotting using a monoclonal GST, a monoclonal PEDF and monoclonal Transportin-SR2 antibody
  22. Samples were then transferred to an airtight chamber maintained at 30uC, and the oxygen content was monitored for at least 5 min
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