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  15. After 15 minutes of washing with an external solution that blocks most NMDAR-mediated synaptic Ca2 influx (5 mM Mg2 /0.6 mM Ca2 )
  16. After 24 h incubation, in HepG2 cells, antiproliferation EC50 for Where Scontrol is the percent of supercoiled DNA in the control lane
  17. After 4 washes, antibody visualization was achieved by the incubation for 3 h at room temperature with Alexa 488-conjugated anti-mouse and/or Alexa 568-conjugated anti-rabbit IgGs
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  41. After approximately 16 hours of incubation with hormone, cells in each well were lysed using 100L mammalian protein extraction reagent
  42. After blocking, sections were stained with antibodies or phalloidin and imaged with a Leica DM4000B fluorescent microscope
  43. After incubation at room temperature for 30 min, liquid of each sample well was removed, washed and subsequently incubated with the color reaction TMB Chromogen in the dark for 15 min at room temperature
  44. After pancreas disgregation, cells were stained with Dithizone to identify the Islets (marked by the arrow) as described in Materials and Methods section
  45. After prediction, the potential miRNA loci were examined carefully based on the distribution and numbers of small RNAs on the entire precursor regions
  46. After reading 18 anti-chicken soup, the brain instantly sober
  47. After recording of the response properties of Vc neurons, lesions were made at the recording site by passing direct current of 20 mA for 15 s
  48. After several failures of in vitro fertilization with ZP-intact oocytes, we performed in vitro fertilization with ZP-free MII oocytes and monitored sperm penetration and PN formation
  49. After that the empiricalbased scoring function was utilized to score and rank all docking answers
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