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  5. After 15 minutes of washing with an external solution that blocks most NMDAR-mediated synaptic Ca2 influx (5 mM Mg2 /0.6 mM Ca2 )
  6. After 24 h incubation, in HepG2 cells, antiproliferation EC50 for Where Scontrol is the percent of supercoiled DNA in the control lane
  7. After 4 washes, antibody visualization was achieved by the incubation for 3 h at room temperature with Alexa 488-conjugated anti-mouse and/or Alexa 568-conjugated anti-rabbit IgGs
  8. After Android App Development: How To Popularize Your App On Google Play
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  31. After approximately 16 hours of incubation with hormone, cells in each well were lysed using 100L mammalian protein extraction reagent
  32. After blocking, sections were stained with antibodies or phalloidin and imaged with a Leica DM4000B fluorescent microscope
  33. After incubation at room temperature for 30 min, liquid of each sample well was removed, washed and subsequently incubated with the color reaction TMB Chromogen in the dark for 15 min at room temperature
  34. After pancreas disgregation, cells were stained with Dithizone to identify the Islets (marked by the arrow) as described in Materials and Methods section
  35. After prediction, the potential miRNA loci were examined carefully based on the distribution and numbers of small RNAs on the entire precursor regions
  36. After reading 18 anti-chicken soup, the brain instantly sober
  37. After recording of the response properties of Vc neurons, lesions were made at the recording site by passing direct current of 20 mA for 15 s
  38. After several failures of in vitro fertilization with ZP-intact oocytes, we performed in vitro fertilization with ZP-free MII oocytes and monitored sperm penetration and PN formation
  39. After that the empiricalbased scoring function was utilized to score and rank all docking answers
  40. After the Storm Guide for Business and Commercial Property Owners
  41. After the viral sequence is acknowledged of a pathogen potentially top to the direct identification of guide compounds
  42. After this time cells were washed with PBS and stimulated with 10% FCS to allow cell recovery for next 18 hrs and later depending on further assays adequately stimulated
  43. After transfection with survivin siRNA, the expression of these mRNAs were significantly decreased in all cell lines as compared to the untransfected control
  44. After treatment with wortmannin at 33 mm for 60 min in the transgenic root tip cells, we observed that the PVC marker GFP-RabF2b was induced to form small vacuoles
  45. After treatments, cells were At the conclusion of experimental treatment, cells in 6-well plates were washed free of media with HBSS, then loaded with the fluorescent dye
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  48. Again-Pain-Dont-Let-It-Management-Your-Lifestyle-v
  49. Again-Soreness-Dont-Enable-It-Manage-Your-Daily-life-d
  50. Against the original House Primarily based Management of Fever Plan (HBMF) upon

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