Некатегоризовані сторінки

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  11. After approximately 16 hours of incubation with hormone, cells in each well were lysed using 100L mammalian protein extraction reagent
  12. After blocking, sections were stained with antibodies or phalloidin and imaged with a Leica DM4000B fluorescent microscope
  13. After incubation at room temperature for 30 min, liquid of each sample well was removed, washed and subsequently incubated with the color reaction TMB Chromogen in the dark for 15 min at room temperature
  14. After pancreas disgregation, cells were stained with Dithizone to identify the Islets (marked by the arrow) as described in Materials and Methods section
  15. After prediction, the potential miRNA loci were examined carefully based on the distribution and numbers of small RNAs on the entire precursor regions
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  22. After this time cells were washed with PBS and stimulated with 10% FCS to allow cell recovery for next 18 hrs and later depending on further assays adequately stimulated
  23. After transfection with survivin siRNA, the expression of these mRNAs were significantly decreased in all cell lines as compared to the untransfected control
  24. After treatment with wortmannin at 33 mm for 60 min in the transgenic root tip cells, we observed that the PVC marker GFP-RabF2b was induced to form small vacuoles
  25. After treatments, cells were At the conclusion of experimental treatment, cells in 6-well plates were washed free of media with HBSS, then loaded with the fluorescent dye
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