Некатегоризовані сторінки

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  1. After approximately 16 hours of incubation with hormone, cells in each well were lysed using 100L mammalian protein extraction reagent
  2. After blocking, sections were stained with antibodies or phalloidin and imaged with a Leica DM4000B fluorescent microscope
  3. After incubation at room temperature for 30 min, liquid of each sample well was removed, washed and subsequently incubated with the color reaction TMB Chromogen in the dark for 15 min at room temperature
  4. After pancreas disgregation, cells were stained with Dithizone to identify the Islets (marked by the arrow) as described in Materials and Methods section
  5. After prediction, the potential miRNA loci were examined carefully based on the distribution and numbers of small RNAs on the entire precursor regions
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  7. After recording of the response properties of Vc neurons, lesions were made at the recording site by passing direct current of 20 mA for 15 s
  8. After several failures of in vitro fertilization with ZP-intact oocytes, we performed in vitro fertilization with ZP-free MII oocytes and monitored sperm penetration and PN formation
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  12. After this time cells were washed with PBS and stimulated with 10% FCS to allow cell recovery for next 18 hrs and later depending on further assays adequately stimulated
  13. After transfection with survivin siRNA, the expression of these mRNAs were significantly decreased in all cell lines as compared to the untransfected control
  14. After treatment with wortmannin at 33 mm for 60 min in the transgenic root tip cells, we observed that the PVC marker GFP-RabF2b was induced to form small vacuoles
  15. After treatments, cells were At the conclusion of experimental treatment, cells in 6-well plates were washed free of media with HBSS, then loaded with the fluorescent dye
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  22. Age-group that we have targeted within a series of studies due
  23. Age and gender do not seem to play distinct role in influencing proliferation rate of degenerative cervical NP cells (data not shown)46105 degenerative cervical NP cell were cultured for four weeks in collagen I scaffold
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  26. Age stratified investigation confirmed no traits in microalbuminuric or macroalbuminuric sufferers (Determine S2)
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  30. Agellomerus 2/length of flagellomerus 14: two.six?.8. Tarsal claws: with single basal spine ike
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