An ATPase Shop Dashboard Widget

Snail exposure to miracidia and maintenance were similar to those in the first experiment. Spring water and food were changed, if necessary, every day until snail death. When the first cercarial shedding occurred, surviving snails were subjected to a thermal shock every three days by placing their Petri dishes at 10��C13?��C for 3?h to stimulate cercarial exit [36, 44]. After their emergence, cercariae were counted and removed from Petri dishes. At the death of each infected snail, Selleckchem Vinorelbine its shell was measured using callipers. Table 2. Snail survival on day 30 post-exposure, prevalence of snail infection, shell growth of infected snails, and number of shed cercariae in four groups of Pseudosuccinea columella exposed to Calicophoron daubneyi. Two hundred snails of each group were exposed ... Data analysis In both experiments, the first two parameters were snail survival on day 30 p.e. and the prevalence of C. daubneyi infection calculated in relation to the number of snails surviving on day 30?p.e. For each parameter, the differences were analyzed using a ��2 test. Two other parameters in the first experiment were the number of free rediae and that of free cercariae ATPase counted in dissected snails. In the second experiment, the shell growth of infected snails at their death, the total number of shed cercariae, and the number of shedding waves for each infected snail [30] were also considered. Individual values recorded for these last five parameters were averaged and standard deviations were established for each Gamma-secretase inhibitor in clinical trial snail group. Normality of these values was analyzed using the Shapiro-Wilk test [39]. According to results given by this test, one-way analysis of variance (ANOVA) or the Kruskal-Wallis test was used to establish levels of significance. In the first experiment, the differences between survival rates, prevalence of infection and the numbers of larval forms were analyzed for each snail population considered separately. In the second experiment, the influence of snail population on the characteristics of C. daubneyi infection was evaluated by comparing differences between the five parameters for each snail group (1 or 2?mm). All statistical analyses were performed using Statview 5.0 software (SAS Institute Inc., Cary, NC, USA). Results Aptitude of P. columella as a snail host for C. daubneyi (experiment 1) In both snail populations (Table 1), the survival rate on day 30 p.e. significantly increased (Egypt: ��2?=?61.28, p?