In mature 3T3-L1 cells, ATGL expression was abolished following TSH treatment in a dose-dependent manner

The epididymal adipose tissue was frozen in learn more liquid nitrogen. (A) The protein expression stages of ATGL in the white adipose tissues of the Tshr-/- mice and Tshr+/+ mice were purchase GW 4064 detected by Western blotting. 4D).In this study, we discovered a novel extra-thyroidal role of TSH in regulating TG metabolism. TSH inhibited ATGL expression, therefore inhibiting basal lipolysis in adipocytes. This activity involved the cAMP/PKA signaling pathway. Our findings indicate a potential role for TSH in the metabolism of TG in adipocytes and they may also aid in the elucidation of the relationship between subclinical hypothyroidism and obesity.Figure 3. TSH decreased ATGL expression in mature differentiated cells. (A) On D12, the cells were treated with 0.1 M bTSH, 1 M bTSH or 2 M bTSH for 24 h or 48 h in serum-starved DMEM. Proteins were separated by SDS-PAGE and immunoblotted for ATGL and GAPDH. Values are quantified by densitometry and normalized with GAPDH. Representative Western blot results are shown. (B) Total RNA was extracted from differentiated cells treated with 2 M bTSH for 48 h in serum-free DMEM. ATGL mRNA levels were determined by real-time PCR and normalized with -actin. Values are reported as the fold change relative to the control group. The data are from 3 independent experiments and are presented as the mean SD. p